Effect of immunostimulating Limosilactobacillus strain in rats with trinitrobenzenesulfonate (TNBS)-induced colitis

Jovanka Lukić; Ivana Strahinić; Marina Milenković; Jelena Begović

doi:10.5937/arhfarm73-46399

Jovanka Lukić Univerzitet u Beogradu – Institut za molekularnu genetiku i genetičko inženjerstvo
Ivana Strahinić Univerzitet u Beogradu – Institut za molekularnu genetiku i genetičko inženjerstvo
Marina Milenković Univerzitet u Beogradu – Farmaceutski fakultet, Katedra za mikrobiologiju i imunologiju
Jelena Begović Univerzitet u Beogradu – Institut za molekularnu genetiku i genetičko inženjerstvo

DOI: https://doi.org/10.5937/arhfarm73-46399

Ključne reči: kolitis, Limosilactobacillus fermentum, probiotik, pro-inflamatorni citokini, lipidna peroksidacija

Sažetak

Cilj ovog istraživanja je bio da se ispita potencijal imunostimulišućeg soja Limosilactobacillus fermentum BGHI14 da smanji oštećenje tkiva debelog creva kod pacova sa kolitisom izazvanim 2,4,6-trinitrobenzensulfonskom kiselinom (TNBS). Pacovi Wistar soja su tretirani sojem L. fermentum BGHI14 u režimu preventivnog, terapijskog i kontinuiranog tretmana 22 dana pre i/ili 7 dana nakon primene TNBS. Nakon žrtvovanja, uzorci debelog creva su sakupljeni za izolaciju RNK, analizu ekspresije gena, histopatološke analize i merenje malondialdehida. Na osnovu telesnih težina pacova, histopatološih rezultata, nivoa malondialdehida i transkripcije IL-1β citokina i proteina tesnih međućelijskih veza (Tjp-1), preventivni i terapijski tretman su se pokazali kao najefikasniji u primenjenim uslovima. S druge strane, kontinuirano lečenje nije uticalo na intenzitet oštećenja tkiva. Uzimajući u obzir ove rezultate, razmotreni su mogući mehanizmi koji stoje iza zaštitnog delovanja imunostimulišućih probiotičkih bakterija u slučaju oštećenja mukozne barijere.

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Efekat imunostimulišućeg soja roda Limosilactobacillus kod pacova sa kolitisom izazvanim trinitrobenzensulfonatom (TNBS)

Sažetak

Reference