Probiotički potencijal sojeva Enterococcus faecium izolovanih iz mlečnih proizvoda sa područja Zapadnog Balkana

  • Nikola Popović Univerzitet u Beogradu – Institut za molekularnu genetiku i genetičko inženjerstvo
  • Amarela Terzić-Vidojević Univerzitet u Beogradu – Institut za molekularnu genetiku i genetičko inženjerstvo
  • Emilija Brdarić Univerzitet u Beogradu – Institut za molekularnu genetiku i genetičko inženjerstvo
  • Svetlana Soković Bajić Univerzitet u Beogradu – Institut za molekularnu genetiku i genetičko inženjerstvo
  • Jelena Djokić Univerzitet u Beogradu – Institut za molekularnu genetiku i genetičko inženjerstvo
  • Milica Živković Univerzitet u Beogradu – Institut za molekularnu genetiku i genetičko inženjerstvo
  • Katarina Veljović Univerzitet u Beogradu – Institut za molekularnu genetiku i genetičko inženjerstvo
DOI: https://doi.org/10.5937/arhfarm73-47047
Ključne reči: enterokoke, faktori virulencije, probiotici, ekstracelularni matriks, adhezija, preživljavanje

Sažetak


Rod Enterococcus je jedan od glavnih rodova koji pripada bakterijama mlečne kiseline i ima kontroverzni status, koji se ogleda u činjenici da su enterokoke prepoznate kao uzročnici bolničkih infekcija, dok se istovremeno koriste i kao probiotici i kao starter kulture. Pripadnici vrste Enterococcus nemaju opštepriznat bezbedni status, niti su uvršteni na liste bezbednih sojeva Evropske agencije za bezbednost hrane. Autohtoni mlečni proizvodi, posebno oni proizvedeni od sirovog mleka, predstavljaju bogate izvore vrsta Enterococcus faecium sa potencijalnim probiotičkim svojstvima. U ovoj studiji je istraživano prisustvo faktora virulencije i sposobnost adhezije vrsta En. faecium izolovanih iz mlečnih, autohtonih proizvoda sa područja Zapadnog Balkana sa ciljem boljeg razumevanja njihovog probiotičkog potencijala, kao i efekata koji doprinose unapređenju zdravlja korisnika.

Reference

Vandera E, Kakouri A, Koukkou A-I, Samelis J. Major ecological shifts within the dominant nonstarter lactic acid bacteria in mature Greek Graviera cheese as affected by the starter culture type. Int J Food Microbiol. 2019; 290:15–26.

Švec P, and Franz CMAP. The family Enterococcaceae. Lactic Acid Bacteria: Biodiversity and Taxonomy. Wiley 2014; p. 171-173

Hanchi H, Mottawea W, Sebei K, Hammami R. The Genus Enterococcus: Between Probiotic Potential and Safety Concerns-An Update. Front Microbiol. 2018;9:1791.

Leclercq R. Epidemiological and resistance issues in multidrug-resistant staphylococci and enterococci. Clin Microbiol Infect Off Publ Eur Soc Clin Microbiol Infect Dis. 2009;15:224–231.

Kiruthiga A, Padmavathy K, Shabana P, Naveenkumar V, Gnanadesikan S, Malaiyan J. Improved detection of esp, hyl, asa1, gelE, cylA virulence genes among clinical isolates of Enterococci. BMC Res Notes. 2020;13:170.

Ramos S, Silva V, Dapkevicius M de LE, Igrejas G, Poeta P. Enterococci, from Harmless Bacteria to a Pathogen. Microorganisms. 2020;8:1118.

Ch’ng J-H, Chong KKL, Lam LN, Wong JJ, Kline KA. Biofilm-associated infection by enterococci. Nat Rev Microbiol. 2019;17:82–94.

Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, et al. Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol. 2014;11:506–514.

Mitra AK, Rabbani GH. A double-blind, controlled trial of bioflorin (Streptococcus faecium SF68) in adults with acute diarrhea due to Vibrio cholerae and enterotoxigenic Escherichia coli. Gastroenterology. 1990;99:1149–1152.

Bybee SN, Scorza AV, Lappin MR. Effect of the probiotic Enterococcus faecium SF68 on presence of diarrhea in cats and dogs housed in an animal shelter. J Vet Intern Med. 2011;25:856–860.

Franz CMAP, Stiles ME, Schleifer KH, Holzapfel WH. Enterococci in foods conundrum for food safety. Int J Food Microbiol. 2003;88:105–122.

Graham K, Stack H, Rea R. Safety, beneficial and technological properties of enterococci for use in functional food applications - a review. Crit Rev Food Sci Nutr. 2020;60:3836–3861.

Terzić-Vidojević A, Veljović K, Popović N, Tolinački M, Golić N. Enterococci from Raw-Milk Cheeses: Current Knowledge on Safety, Technological, and Probiotic Concerns. Foods Basel Switz. 2021;10:2753.

Popović N, Dinić M, Tolinački M, Mihajlović S, Terzić-Vidojević A, Bojić S, et al. New Insight into Biofilm Formation Ability, the Presence of Virulence Genes and Probiotic Potential of Enterococcus sp. Dairy Isolates. Front Microbiol. 2018;9:78.

Kainulainen V, Korhonen TK. Dancing to another tune-adhesive moonlighting proteins in bacteria. Biology. 2014;3:178–204.

Somarajan SR, La Rosa SL, Singh KV, Roh JH, Höök M, Murray BE. The fibronectin-binding protein Fnm contributes to adherence to extracellular matrix components and virulence of Enterococcus faecium. Infect Immun. 2015;83:4653–4661.

Pinkston KL, Gao P, Diaz-Garcia D, Sillanpää J, Nallapareddy SR, Murray BE, Harvey BR. The Fsr quorum-sensing system of Enterococcus faecalis modulates surface display of the collagen-binding MSCRAMM Ace through regulation of gelE. J Bacteriol. 2011;193:4317–4325.

Garsin DA, Frank KL, Silanpää J, Ausubel FM, Hartke A, Shankar N, Murray BE. Pathogenesis and Models of Enterococcal Infection. In: Gilmore, MS, Clewell, DB, Ike, Y, Shankar, N (eds.), Enterococci: From Commensals to Leading Causes of Drug Resistant Infection. Massachusetts Eye and Ear Infirmary, Boston2014; (internet).

Parish, JH. Genetic manipulation of streptomyces—A laboratory manual: By DA Hopwood, MJ Bibb, KF Chater; T Kieser CJ Bruton, HM Kieser, DJ Lydiate, CP Smith, JM Ward and H Schrempf. pp 356. The John Innes Foundation, Norwich, UK and Cold Spring Harbour Laboratory. 1985. ISBN 0‐7084‐0336‐0. 1986; 196-196.

Lesuffleur T, Barbat A, Dussaulx E, Zweibaum A. Growth adaptation to methotrexate of HT-29 human colon carcinoma cells is associated with their ability to differentiate into columnar absorptive and mucus-secreting cells. Cancer Res. 1990;50(19):6334-43.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65:55–63.

Popović N, Brdarić E, Đokić J, Dinić M, Veljović K, Golić N, Terzić-Vidojević A. Yogurt Produced by Novel Natural Starter Cultures Improves Gut Epithelial Barrier In Vitro. Microorganisms. 2020;8:1586.

Valeriano VD, Parungao-Balolong MM, Kang D-K. In vitro evaluation of the mucin-adhesion ability and probiotic potential of Lactobacillus mucosae LM1. J Appl Microbiol. 2014;117:485–497.

Živković M, Miljković MS, Ruas-Madiedo P, Markelić MB, Veljović K, Tolinački M, et al. EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp. paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E. coli Association to Caco-2 Cells. Front Microbiol. 2016;7:286.

Terzić-Vidojević A, Veljović K, Begović J, Filipić B, Popović D, Tolinački M, et al. Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures? Front Microbiol. 2015;6:954.

Anderson AC, Jonas D, Huber I, Karygianni L, Wölber J, Hellwig E, et al. Enterococcus faecalis from Food, Clinical Specimens, and Oral Sites: Prevalence of Virulence Factors in Association with Biofilm Formation. Front Microbiol. 2015;6:1534.

Fisher K, Phillips C. The ecology, epidemiology and virulence of Enterococcus. Microbiol Read Engl. 2009;155:1749–1757.

Marco ML, Pavan S, Kleerebezem M. Towards understanding molecular modes of probiotic action. Curr Opin Biotechnol. 2006;17:204–210.

Revolledo L, Ferreira AJP, Mead GC. Prospects in Salmonella Control: Competitive Exclusion, Probiotics, and Enhancement of Avian Intestinal Immunity. J Appl Poult Res. 2006;15:341–351.

Howarth GS, Wang H. Role of endogenous microbiota, probiotics and their biological products in human health. Nutrients. 2013;5:58–81.

Sánchez J-I, Martínez B, Guillén R, Jiménez-Díaz R, Rodríguez A. Culture conditions determine the balance between two different exopolysaccharides produced by Lactobacillus pentosus LPS26. Appl Environ Microbiol. 2006;72:7495–7502.

Zareba TW, Pascu C, Hryniewicz W, Wadström T. Binding of extracellular matrix proteins by enterococci. Curr Microbiol. 1997;34:6–11.

Miljkovic M, Strahinic I, Tolinacki M, Zivkovic M, Kojic S, Golic N, Kojic M. AggLb Is the Largest Cell-Aggregation Factor from Lactobacillus paracasei сubsp. paracasei BGNJ1-64, Functions in Collagen Adhesion, and Pathogen Exclusion In Vitro. PloS One. 2015;10:e0126387.

Veljović K, Popović N, Miljković M, Tolinački M, Terzić-Vidojević A, Kojić M. Novel Aggregation Promoting Factor AggE Contributes to the Probiotic Properties of Enterococcus faecium BGGO9-28. Front Microbiol. 2017;8:1843.

Probiotics in food: health and nutritional properties and guidelines for evaluation ; report of a Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic Acid Bacteria, Córdoba, Argentina, 1 - 4 October 2001 ; report of a Joint FAO/WHO Working Group on Drafting Guidelines for the Evaluation of Probiotics in Food, London, Ontario, Canada, 30 April - 1 May 2002. Rome: Food and Agriculture Organization of the United Nations [u.a], 2006.

Nakayama J, Kariyama R, Kumon H. Description of a 23.9-kilobase chromosomal deletion containing a region encoding fsr genes which mainly determines the gelatinase-negative phenotype of clinical isolates of Enterococcus faecalis in urine. Appl Environ Microbiol. 2002;68:3152–5.

Duprè I, Zanetti S, Schito AM, Fadda G, Sechi LA. Incidence of virulence determinants in clinical Enterococcus faecium and Enterococcus faecalis isolates collected in Sardinia (Italy). J Med Microbiol. 2003;52:491–498.

Vankerckhoven V, Van Autgaerden T, Vael C, Lammens C, Chapelle S, Rossi R, et al. Development of a multiplex PCR for the detection of asa1, gelE, cylA, esp, and hyl genes in enterococci and survey for virulence determinants among European hospital isolates of Enterococcus faecium. J Clin Microbiol. 2004;42:4473–9.

Eaton TJ, Gasson MJ. Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates. Appl Environ Microbiol. 2001;67:1628–35.

Objavljeno
2023/12/23
Broj časopisa
Vol. 73 Br. Notebook 6 (2023): Arhiv za farmaciju
Rubrika
Originalni naučni rad
Autori koji objavljuju u ovom časopisu pristaju na sledeće uslove:

  1. Autori zadržavaju autorska prava i pružaju časopisu pravo prvog objavljivanja rada i licenciraju ga "Creative Commons Attribution licencom" koja omogućava drugima da dele rad, uz uslov navođenja autorstva i izvornog objavljivanja u ovom časopisu.

  2. Autori mogu izraditi zasebne, ugovorne aranžmane za neekskluzivnu distribuciju članka objavljenog u časopisu (npr. postavljanje u institucionalni repozitorijum ili objavljivanje u knjizi), uz navođenje da je članak izvorno objavljen u ovom časopisu.

  3. Autorima je dozvoljeno i podstiču se da postave objavljeni članak onlajn (npr. u institucionalni repozitorijum ili na svoju internet stranicu) pre ili tokom postupka prijave rukopisa, s obzirom da takav postupak može voditi produktivnoj razmeni ideja i ranijoj i većoj citiranosti objavljenog članka (Vidi Efekti otvorenog pristupa).