MARKERS OF INFLAMMATION AND IMMUNOLOGICAL COMPETENCE: ASSESSMENT IN THE EARLY POSTOPERATIVE PHASE OF CARDIAC SURGERY INVOLVING EXTRACORPOREAL CIRCULATION:

Selma Mutevelić; Lejla Bajramović-Omeragić; Merima Šehić; Sumejja Baljević-Spahić; Belma Pehlivanović Kelle; Ermin Begović; Berina Hasanefendić; Ermina Mujičić; Marina Delić-Šarac; Drago Batinić

doi:10.5937/jomb0-56889

Selma Mutevelić Department of Clinical Immunology, Clinical Center University of Sarajevo, Sarajevo, Bosnia and Herzegovina https://orcid.org/0009-0000-8967-4316
Lejla Bajramović-Omeragić Department of Medical Biochemistry and Immunology Diagnostics, County Hospital Zenica, Zenica, Bosnia and Herzegovina https://orcid.org/0000-0002-7851-6440
Merima Šehić Department of Clinical Immunology, Clinical Center University of Sarajevo, Sarajevo, Bosnia and Herzegovina https://orcid.org/0000-0002-4829-1623
Sumejja Baljević-Spahić Department of Clinical Pathology, Cytology and Human Genetics, Clinical Center University of Sarajevo, Sarajevo, Bosnia and Herzegovina https://orcid.org/0000-0003-2802-8171
Belma Pehlivanović Kelle Department of Pharmacology and Clinical Pharmacy, University of Sarajevo - Faculty of Pharmacy https://orcid.org/0000-0002-1574-493X
Ermin Begović Department of Clinical Biochemistry, Clinical Centre University of Sarajevo, Sarajevo, Bosnia and Herzegovina https://orcid.org/0000-0003-2802-8171
Berina Hasanefendić Department of Clinical Biochemistry, Clinical Centre University of Sarajevo, Sarajevo, Bosnia and Herzegovina https://orcid.org/0000-0002-9610-5020
Ermina Mujičić Clinic of Cardiovascular Surgery, Clinical Centre University of Sarajevo, Sarajevo, Bosnia and Herzegovina https://orcid.org/0000-0003-2802-8171
Marina Delić-Šarac Oslo University Hospital Ullevål, Clinic for Laboratory Medicine, Immunology and Transfusion Medicine, Oslo, Norway https://orcid.org/0000-0002-3939-8197
Drago Batinić University of Zagreb School of Medicine, Zagreb, Croatia https://orcid.org/0000-0001-5410-0195

DOI: https://doi.org/10.5937/jomb0-56889

Keywords: extracorporeal circulation, C-reactive protein, serum amyloid A, monocytes

Abstract

Background: To gain insight into the role and relevance of inflammatory and immunological markers in the comprehensive assessment of a patient’s immune response to surgical procedures. This study focused on investigating preoperative and postoperative serum levels dynamics of SAA, CRP, and proportion of HLA-DR⁺CD14⁺monocytes, CD14⁺ monocytes, and pro-inflammatory monocytes CD16⁺CD14⁺ in patients who underwent heart surgery using extracorporeal circulation (on-pump).

Methods: An observational, prospective study was conducted at the Heart Center of the Clinical Center of the University of Sarajevo on 53 patients divided into 3 age groups: 50 – 59, 60 – 69, and 70 – 80. The serum levels of CRP and SAA were quantitatively determined by immunonephelometry. At the same time, flow cytometry technology was applied to measure the proportion of CD14⁺ monocytes, HLA-DR⁺CD14⁺monocytes, and pro-inflammatory CD16⁺CD14⁺ monocytes.

Results: Measured values of CRP, SAA, proportion of monocytes CD14⁺, and proportion of pro-inflammatory monocytes CD16⁺CD14⁺ are significantly increased postoperatively compared to the preoperative values (p<0.05). The proportion of HLA-DR⁺CD14⁺ monocytes is lower postoperatively compared to preoperative values (p<0.001). Furthermore, there are no significant gender differences in the preoperative or postoperative parameters (p>0.05), with the notable exception of the preoperative proportion of CD14⁺monocytes (p<0.05). The analysis of age-related differences indicates no significant changes in the observed preoperative and postoperative parameters among the defined age groups (p>0.05).

Conclusion: Early monitoring of inflammatory and immunological markers in the postoperative phase could be valuable for healthcare professionals to implement prompt interventions to mitigate negative outcomes.

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MARKERS OF INFLAMMATION AND IMMUNOLOGICAL COMPETENCE: ASSESSMENT IN THE EARLY POSTOPERATIVE PHASE OF CARDIAC SURGERY INVOLVING EXTRACORPOREAL CIRCULATION

Markers of inflammation and immunological competence

Abstract

References