CRUDE GLYCEROL FROM BIODIESEL INDUSTRY AS FEEDSTOCK FOR XANTHAN PRODUCTION

  • Nikolina Danilović University of Novi Sad, Faculty of Technology Novi Sad
Keywords: waste utilization, crude glycerol, xanthan production, Xanthomonas campestris

Abstract


A rapid expansion of the biodiesel industry has created various ecological issues relative to crude glycerol disposal. Xanthan biosynthesis is considered one of the sustainable solutions for minimizing the adverse effects of waste crude glycerol on the environment. The initial phase of xanthan production on crude glycerol entails the screening of producing microorganism. Therefore, the purpose of this study is to examine the possibility of xanthan production on a crude glycerol-based medium using different Xanthomonas campestris strains. The bioprocesses performed were assessed according to the rheology of the media considered, amounts of xanthan produced and conversion degrees of the most important nutrients present. The pseudoplastic behavior of all the media considered, the amounts of xanthan produced (5.22-7.67 g/L) and the degrees of crude glycerol, total nitrogen and phosphorus conversion (34.44-57.61 %, 23.04-30.35 % and 18.20-22.28 %, respectively) suggest that crude glycerol, after  additional bioprocess optimization, can be a suitable raw material for the industrial production of xanthan.

References

Björn, Annika., Karlsson, Anna., Svensson, B.H., Ejlertsson, J., de La Monja, Paula Segura. (2012). Rheological characterization. INTECH Open Access Publisher.
Brandão, L.V., Assis, D.J., López, J.A., Espiridião, M.C.A., Echevarria, E.M., Druzian, Janice I. (2013). Bioconversion from crude glycerin by Xanthomonas campestris 2103: xanthan production and characterization. Brazilian Journal of Chemical Engineering, 30 (4), 737-746.
da Silva, G. P., Mack, M., Contiero, J. (2009). Glycerol: A promising and abundant carbon source for industrial microbiology. Biotechnology Advances, 27(1), 30-39.
de Jesus Assis, D., Brandão, Líllian Vasconcelos, de Sousa Costa, Larissa Alves, Figueiredo, T.V.B., Sousa, Luciane Santos, Padilha, F.F., Druzian, Janice I.. (2014). A study of the effects of aeration and agitation on the properties and production of xanthan gum from crude glycerin derived from biodiesel using the Response Surface Methodology. Applied Biochemistry and Biotechnology, 172, 2769-2785.
Gales, M.E.Jr., Julian, E.C., Kroner, R.C. (1966). Method for quantitative determination of total phosphorus in water. Journal - American Water Works Association, 58, 1363-1368.
Garcia-Ochoa, F., Santos, Victoria, Casas, J.A., Gómez, E. (2000). Xanthan gum: Production, recovery, and properties. Biotechnology Advances, 18, 549–579.
He, Quan, McNutt, J., Yang, J. (2017). Utilization of the residual glycerol from biodiesel production for renewable energy generation. Renewable and Sustainable Energy Reviews, 71(C), 63-76.
Hejna, A., Kosmela, Paulina, Formela, K., Piszczyk, L., Haponiuk, J. T. (2016). Potential applications of crude glycerol in polymer technology–Current state and perspectives. Renewable and Sustainable Energy Reviews, 66(C), 449-475.
Helrich, K. (1990). Official Methods of Analysis of the Association of Official Analytical Chemists, 5th edn., Association of Official Analytical Chemists, Arlington.
Johnson, D. T., Taconi, Katherine. (2007). The glycerin glut: Options for the value‐added conversion of crude glycerol resulting from biodiesel production. Environmental Progress, 26 (4), 338-348.
Kumara, S.M., Khan, B.A., Rohit, K.C., Purushotham, B. (2012). Effect of carbon and nitro-gen sources on the production of xanthan gum from Xanthomonas campestris isolated from soil. Archives of Applied Science Research, 4 (6), 2507-2512.
Petri, D.F.S. (2015). Xanthan Gum: A versatile biopolymer for biomedical and technological applications. Journal of Applied Polymer Science, 132 (23), 42035.
Quispe, C.A.G., Coronado, C.J.R., Carvalho, J.J.A. (2013). Glycerol: Production, consumption, prices, characterization and new trends in combustion. Renewable and Sustainable Energy Reviews, 27(C), 475-493.
Reis, Elisiane C., Almeida, Monica, Cardoso, Juliana C., Pereira, Maysa de A., de Oliveira, C.B.Z., Venceslau, Emanuella M., Druzian, Janice I., Mariano, Rosa, Padilha, Francine F. (2010). Biopolymer synthesized by strains of Xanthomonas sp. isolate from Brazil using biodiesel-waste. Macromolecular Symposia, 296 (1), 347-353.
Roseiro, J.C., Esgalhado, M.E., Amaral Collaço, M. T., Emery, A. N. (1992). Medium development for xanthan production. Process Biochemistry, 27, 167-175.
Zahović, Ida, Rončević, Zorana, Dodić, S., Grahovac, Jovana, Dodić Jelena (2019). Ispitivanje mogućnosti iskorišćenja otpadnog glicerola u biotehnološkoj proizvodnji ksantana: VII Memorijalni naučni skup iz zaštite životne sredine „Docent dr Milena Dalmacija“, Novi Sad, Srbija, UO-04.
Published
2020/06/15
Section
Papers