Ultrastructural morphometry of neurosecretory granules in neuroblastomas of pediatric patients
Sažetak
Introduction: Peripheral neuroblastic tumours are among the most frequently detected tumours in paediatric patients. A correlation between the aggressiveness of these tumours and their clinical prognosis has been discovered. Neurosecretory granules containing neurotransmitters, an ultrastructural feature of neurons, were found in the cells of peripheral neuroblastic tumours, as well.
Aim: The aim of this study was to compare the number of neurosecretory granules found in cells of tumours with a favourable prognosis to the corresponding number in cells of tumours with an unfavourable one with the use of transmission electron microscopy (TEM).
Material and Methods: Ten tissue biopsies were obtained from paediatric patients diagnosed with peripheral neuroblastic tumours. The tumour samples were first frozen, then fixed in glutaraldehyde, and embedded in epoxy resin. Ultrastructural morphometric analysis was performed on ultrathin sections.
Results: It was found that the cells of tumours with an unfavourable prognosis have more neurosecretory granules in their cytoplasm than the cells of tumours with a favourable one.
Conclusion: The larger number of neurosecretory granules found in cells of tumours with an unfavourable prognosis could potentially shed some light on the role these structures play in tumourigenesis.
Key words: neurosecretory granules, peripheral neuroblastic tumours, prognosis, ultrastructural morphometry
Reference
(1)Anđelković Z, Somer Lj, Avramović V, Milosavljević Z, Tanasković I, Matavulj M,
(2)Samardzija G, Kravić-Stevović T, Đuričić S, Đokić D, Đurišić M, Ćirić D, Martinović
(3) Parham D. Neuroectodermal and Neuroendocrine Tumours Principally Seen in Children.
(4)Romansky S, Crocker D, Shaw K. Ultrastructural Studies on Neuroblastoma (Evaluation
(5)Bumbaširević V, Lačković V, Milićević N.M, Milićević Ž, Mujović S, Obradović M,
(6)Luksch R, Castellani MR, Collini P, De Bernardi B, Conte M, Gambini C, Gandola L, Garaventa A, Biasoni D, Podda M, Sementa AR, Gatta G, Tonini GP. Neuroblastoma (Peripheral neuroblastictumours).Critical Reviews in Oncology/Hematology. 2016;107:163-181.
(7) Latimer E, Anderson G, Sebire NJ. Ultrastructural features of neuroblastictumours in relation to morphological, and molecular findings; a retrospective review study. BMC Clinical
(8) Morris JF, Cannata MA.Ultrastructural preservation of the dense core of posterior pituitary neurosecretory granules and its implications for hormone release.Journal of Endocrinology. 1973; 57:517–529
(9)Cohn SL, Pearson AD, London WB, Monclair T, Ambros PF, Brodeur GM, Faldum A, Hero B, Iehara T, Machin D, Mosseri V, Simon T, Garaventa A, Castel V, Matthay KK; INRG Task Force. The International Neuroblastoma Risk Group (INRG) classification system: an INRG Task Force report. Journal of Clinical Oncology.2009; 27:289-297.
(10) Nakazawa A, Haga C, Ohira M, Okita H, Kamijo T, Nakagawara A. Correlation between the International Neuroblastoma Pathology Classification and genomic signature in neuroblastoma. Cancer Science.2015; 106:766-771.
(11)Gutiérrez LM, Villanueva J. The role of F-actin in the transport and secretion of chromaffin granules: an historic perspective. Pflugers Archive: the European Journal of Physiology. 2017 Jul 20. doi: 10.1007/s00424-017-2040-9. [Epub ahead of print]
(12)Klionski D et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. 2016; 12:1-222.