UTICAJ HRONIČNOG IMOBILIZACIONOG STRESA NA ESTRUSNI CIKLUS MIŠICA SOJA NMRI

Jelena Ristić; Damir Bogdan; Pavle Banović

doi:10.5937/mp72-28778

Jelena Ristić Medicinski fakultet Novi Sad, Univerzitet u Novom Sadu
Damir Bogdan Departman za psihologiju, Filozofski fakultet, Univerzitet u Novom Sadu
Pavle Banović 1. Katedra za mikrobiologiju sa parazitologijom i imunologijom, Medicinski fakultet Novi Sad, Univerzitet u Novom Sadu http://orcid.org/0000-0001-7902-6606

DOI: https://doi.org/10.5937/mp72-28778

Ključne reči: stres, estrusni ciklus, miš, soj NMRI

Sažetak

Uvod: Stres je termin kojim označavamo fenomene prisutne na različitim nivoima biološke organizacije indukovane događajima koji se nazivaju stresori. Stresor može biti objektivna opasnost ili percepcija određene situacije kao opasnost po homeostazu organizma. Hronični stres je kao etiološki faktor u nastanku bolesti doveden u vezu sa reproduktivnim zdravljem žena, pre svega sa poremećajima menstrualnog ciklusa.

Cilj: Ispitati efekat hroničnog imobilizacionog stresa na estrusni ciklus mišica soja NMRI.

Materijal i metode: 12 polno zrelih ženki miša soja NMRI je nasumično podeljeno u kontrolnu i eksperimentalnu grupu. Eksperimentalnoj grupi je indukovan hronični imobilizacioni stres 2 sata dnevno tokom 14 dana. Svakodnevno je svim životinjama uziman vaginalni ispirak. Ispirci su služili za pravljenje razmaza, koji su ispitivani na svetlosnom mikroskopu. Analiza preparata je obuhvatala određivanje faza estrusnog ciklusa.

Rezultati: Kod mišica je uočen estrusni ciklus koji se sastoji iz četiri faze: proestrus, estrus, metestrus i diestrus. U adaptacionom periodu, zapažen je prolongirani diestrus, dominantno prisutan u obe grupe. U periodu indukovanja stresa, kod kontrolne grupe je uočena kontinuirana diestrusna faza, dok je kod eksperimentalne grupe uočen izlazak iz diestrusa i prisustvo drugih faza u ciklusu. Druge faze su posmatrane kao odstupanje od diestrusa. Hi kvadrat testom je utvrđeno da je frekvencija odstupanja statistički značajna(p<0.01).

Zaključak: Indukovanje imobilizacionog stresa dovodi do izlaska životinja iz diestrusa i velikih varijacija u fazama estrusnog ciklusa mišica soja NMRI. S obzirom da mišice soja NMRI dele isti hormonski mehanizan regulacije estrusnog ciklusa, kao žene menstrualnog, postoji mogućnost da bi one mogle da predstavljaju dobar model za proučavanje reproduktivnih poremećaja žena.

Biografija autora

Pavle Banović, 1. Katedra za mikrobiologiju sa parazitologijom i imunologijom, Medicinski fakultet Novi Sad, Univerzitet u Novom Sadu

2. afilijacija:

Pasterov zavod Novi Sad

Pasteur Institute Novi Sad

Reference

(1) Del Giudice M, Buck CL, Chaby LE, Gormally BM, Taff CC, Thawley CJ, et al. What Is Stress? A Systems Perspective. Integr Comp Biol. 2018;58(6):1019-1032.
(2) Ubaldi M, Ricciardelli E, Pasqualini L, Sannino G, Soverchia L, Ruggeri B, et al. Biomarkers of hippocampal gene expression in a mouse restraint chronic stress model.Pharmacogenomics. 2015;16(5):471-82.
(3) Belanoff JK, Gross K, Yager A, Schatzberg AF. Corticosteroids and cognition. J Psychiatr Res. 2001;35(3):127-45.
(4) Verma R, Balhara YP, Gupta CS. Gender differences in stress response: Role of developmental and biological determinants.Ind Psychiatry J. 2011;20(1):4-10.
(5) Allsworth JE, Clarke J, Peipert JF, Hebert MR, Cooper A, Boardman LA. The influence of stress on the menstrual cycle among newly incarcerated women. Womens Health Issues. 2007;17(4):202-9.
(6) Shors TJ, Chua C, Falduto J. Sex differences and opposite effects of stress on dendritic spine density in the male versus female hippocampus. J Neurosci. 2001;21(16):6292-7.
(7) Westenbroek C, Den Boer JA, Veenhuis M, Ter Horst GJ. Chronic stress and social housing differentially affect neurogenesis in male and female rats. Brain Res Bull. 2004;64(4):303-8.
(8) Xu M, Sun J, Wang Q, Zhang Q, Wei C, Lai D. Chronic restraint stress induces excessive activation of primordial follicles in mice ovaries. PLoS One. 2018;13(3):e0194894.
(9) Wu LM, Liu YS, Tong XH, Shen N, Jin RT, Han H, et al. Inhibition of Follicular Development Induced by Chronic Unpredictable Stress Is Associated with Growth and Differentiation Factor 9 and Gonadotropin in Mice. Biol Reprod. 2012;86(4):121:1-7.
(10) Ferin M. Clinical review 105: Stress and the reproductive cycle. J Clin Endocrinol Metab. 1999;84(6):1768-74.
(11) Dadomo H, Gioiosa L, Cigalotti J, Ceresini G, Parmigiani S, Palanza P. What is stressful for females? Differential effects of unpredictable environmental or social stress in CD1 female mice. Horm Behav. 2018;98:22-32.
(12) Sutanto W, de Kloet ER. The use of various animal models in the study of stress and stress-related phenomena. Lab Anim. 1994;28(4):293-306.
(13) Smieško G, Banović P, Gusman V, Simin V, Cimpean AM, Lalošević D. Molecular evaluation of chronic restrain stress in mice model of non metastatic fibrosarcoma. J Mol Hist. 2020;51:367–374.
(14) Fedrigoli E, Bogdan D, Lalošević D, Banović P. Proposition of a simplified protocol and new parameter introduction In NMRI mice anhedonia induction. Serbian J Exp Clin Res. 2020. In press. https://doi.org/10.2478/sjecr-2020-0021.
(15) Cora MC, Kooista L, Travlos G. Vaginal Cytology of Laboratory Rat and Mouse: Review and Criteria for Staging of the Estrous Cycle Using Stained Vaginal Smears. Toxologic Pathology. 2015;43(6):776-93.
(16) Popovic M, Popovic N. Int J Psychophysiol. Estrus cycle and gastric lesions in individual- and group-stressed female rats. 1999;33(1):21-6.
(17) Mesa-Gresa P, Ramos-Campos M, Redolat R. Corticosterone levels and behavioral changes induced by simultaneous exposure to chronic social stress and enriched environments in NMRI male mice. Physiol Behav. 2016;158:6-17.
(18) Ryan KD, Schwartz NB. Grouped female mice: demonstration of pseudopregnancy. Biol Reprod. 1977;17(4):578-83.
(19) Engeland WC, Massman L, Miller L, Leng S, Pignatti E, et al. Sex Differences in Adrenal Bmal1 Deletion-Induced Augmentation of Glucocorticoid Responses to Stress and ACTH in Mice. Endocrinology. 2019;160(10):2215-2229.
(20) Stephens MA, Mahon PB, McCaul ME, Wand GS. Hypothalamic‐pituitary‐adrenal axis response to acute psychosocial stress: Effects of biological sex and circulating sex hormones. Psychoneuroendocrinology. 2016;66:47-55.
(21) Tarín JJ, Hamatani T, Cano A. Acute stress may induce ovulation in women. Reprod Biol Endocrinol. 2010;8:53:1-13.
(22) Oyola MG, Handa RJ. Hypothalamic–pituitary–adrenal and hypothalamic–pituitary–gonadal axes: sex differences in regulation of stress responsivity. Stress. 2017;20(5):476-494.
(23) Prokai D, Berga SL. Neuroprotection via Reduction in Stress: Altered Menstrual Patterns as a Marker for Stress and Implications for Long-Term Neurologic Health in Women. Int J Mol Sci. 2016;17(12):2147.
(24) Phumsatitpong C, Moenter SM. Estradiol-Dependent Stimulation and Suppression of Gonadotropin-Releasing Hormone Neuron Firing Activity by Corticotropin-Releasing Hormone in Female Mice. Endocrinology. 2018;159(1):414-425.