Etiologija bakterioznih oboljenja mladih zasada oraha u Srbiji

  • Renata Iličić University of Novi Sad, Faculty of Agriculture, Trg Dositeja Obradovića 8, Novi Sad, Serbia
  • Ferenc Bagi University of Novi Sad, Faculty of Agriculture, Trg Dositeja Obradovića 8, Novi Sad, Serbia
  • Milan Blagojević Academy of Professional Studies, Dobropoljska 5, Šabac, Serbia
  • Jovica Gošić University of Novi Sad, Faculty of Agriculture, Trg Dositeja Obradovića 8, Novi Sad, Serbia
  • Predrag Milovanović Agrosava doo, Palmira Toljatija 5/IV, Belgrade, Serbia
  • Tatjana Popović Institut za zaštitu bilja i životnu sredinu, Beograd
Ključne reči: orah, fitopatogene bakterije, vertikalni rak, identifikacija patogena

Sažetak


Tokom leta i jeseni u periodu 2019-2020, praćeno je prisustvo bakterioznih oboljenja u mladim zasadima oraha. Oboleli uzorci stabala i grana sa simptomima vertikalnog raka (VOC), bakteriozne plamenjače (WBB) i površinske nekroze kore prikupljeni su sa osam lokaliteta u Srbiji. Na osnovu fenotipskih karakteristika, patogenosti i molekularnih testova korišćenjem PCR-a i specifičnih prajmera, 49 izolata sa simptomima VOC i WBB je identifikovano kao Xanthomonas arboricola pv. juglandis i dva izolata sa simptoma površinske nekroze kore identifikovana su kao Brenneria rubrifaciens. Izolat X. a. pv. juglandis dobijen iz VOC simptoma na inokulisanim stablima mladih sadnica oraha (sorte Chandler, Frankquette i Šejnovo) je prouzrokovao duboke rak rane na kori. Ova istraživanja daju prve rezultate o povezanosti X. a. pv. juglandis sa VOC simptomоm u Srbiji. S obzirom da X. a. pv. juglandis značajno ugrožava proizvodnju oraha preporučuje se da se testiranje na prisustvo ovog patogena vrši prilikom uvoza sadnica od strane ovlašćene laboratorije. Dalje, rezultati ove studije predstavljaju i prvi dokaz o prisustvu B. rubrifaciens na orahu u Srbiji, ali je utvrđeno da patogen ima manji uticaj na mlada stabla oraha.

Biografija autora

Tatjana Popović, Institut za zaštitu bilja i životnu sredinu, Beograd
Srbija

Reference

Akca, Y., & Yilmaz, S. (2017). Walnut production in Turkey from past to present. In Kovačević, D. (ed.), Book of proceedings VIII International Scientific Agricultural Symposium “Agrosym 2017”. Jahorina, Sarajevo, Bosnia and Herzegovina: East Sarajevo: Faculty of Agriculture.

 

Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., & Struhl, K. (2003). In Current protocols in molecular biology. (Vol. 1, pp 208-212). New York, USA: John Wiley.

 

Biosca, E.G., Gonzalez, R., Lopez-Lopez, M.J., Soria, S., Monton, C., Perez-Laorga, E., & Lopez M.M. (2003). Isolation and characterization of Brenneria quercina causal agent for bark canker and drippy nut of Quercus spp. in Spain. Phytopathology, 93, 485-492.

 

Biosca, E.G., & Lopez, M.M. (2012). Detection and identification methods and new tests as developed and used in the framework of COST 873 for bacteria pathogenic to stone fruits and nuts, Brenneria nigrifluens and Brenneria rubrifaciens. Journal of Plant Pathology, 94 (1, Supplement), S1.105-S1.113.

 

Buchner, R.P., Gilles, C., Olson, W.H., Adaskaveg, J.E., Lindow, S.E., & Koutsoukis, R. (2010). Spray timing and materials for walnut blight (Xanthomonas campestris pv. juglandis, xanthomonas arboricola pv. juglandis) control in Northern California USA. Acta Horticulturae, 861, 457-464.

 

Buchner, R.P., Lindow, S.E., Adaskaveg, J.E., Gilles, C.K., & Koutsoukis, R. (2012). Walnut blight control using integrated pest management techniques. Walnut Research Reports, 329-344. Available at: http://walnutresearch.ucdavis.edu/2012/2012_329.pdf.

 

Fernandes, C., Blom, J., Pothier, J.F., & Tavares, F. (2018). High-quality draft genome sequence of Xanthomonas arboricola pv. juglandis CPBF 1521, isolated from leaves of a symptomatic walnut tree in Portugal without a past of phytosanitary treatment. Microbiology Resource Announcements, 7(16), e00887-18.

 

Freschi, C.R., Silva Carvalho, L.F.O., & Oliveira, C.J.B. (2005). Comparison of DNA-extraction methods and selective enrichment broths on the detection of Salmonella Typhimurium in swine feces by polymerase chain reaction (PCR). Brazilian Journal of Microbiology, 36, 363-367.

 

Frutos, D. (2010). Bacterial diseases of walnut and hazelnut and genetic resources. Journal of Plant Pathology, 92 (1, Supplement), S1.79-S1.85.

 

Gavrilović, V., & Arsenijević, M. (1998). Etiological study of bacterial spots of walnut fruits. Zaštita bilja/Plant Protection, 49, 295-302. (in Serbian)

 

Giovanardi, D., Bonneau, S., Gironde, S., Fischer-Le Saux, M., Manceau, C., & Stefani, E. (2016). Morphological and genotypic features of Xanthomonas arboricola pv. juglandis populations from walnut groves in Romagna region, Italy. European Journal of Plant Pathology, 145, 1-16.

 

Gironde, S., Guillaumes, J. & Manceau, C. (2009). Specific detection of Xanthomonas arboricola pv. juglandis pathogen on walnut. In EPPO Conference on Diagnostics (pp 1. York, UK: Food and Environment Research Agency.

 

Hajri, A., Meyer, D., Delort, F., Guillaumes, J., Brin, C., & Manceau, C. (2010). Identification of a genetic lineage within Xanthomonas arboricola pv. juglandis as the causal agent of vertical oozing canker of Persian (English) walnut in France. Plant Pathology, 59, 1014-1022.

 

Ivanović, Ž., Popović, T., Janse, J., Kojić, M., Stanković, S., Gavrilović, V., & Fira, Đ. (2015). Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques. European Journal of Plant Pathology, 141, 133-145.

 

Kim, H. S., Cheon, W., Lee, Y., Kwon, H. T., Seo, S. T., Balaraju, K., & Jeon, Y. (2021). Identification and characterization of Xanthomonas arboricola pv. juglandis causing bacterial blight of walnuts in Korea. Plant Pathology Journal, 37(2), 137-151.

 

Lang, M.D., & Evans, K.J. (2010). Epidemiology and status of walnut blight in Australia. Journal of Plant Pathology, 92 (1, Supplement), Sl.49-Sl.55.

 

Lindow, S., Olson, W., & Buckner, R. (2014). Colonization of dormant walnut buds by Xanthomonas arboricola pv. juglandis is predictive of subsequent disease. Phytopathology, 104, 1163-1174.

 

Loreti, S., De Simone, D., & Gallelli, A. (2008). Detection and identification of Brenneria nigrifluens, the causal agent of the shallow bark canker of walnut by PCR amplification. Journal of Phytopathology, 156, 464-469.

 

McClean, A.E., & Kluepfel, D.A. (2009). Genetic loci involved in rubrifacine production in the walnut pathogen Brenneria rubrifaciens. Phytopathology, 99, 145-151.

 

McClean, A.E., Sudarshana, P., & Kluepfel, D.A. (2008). Enhanced detection and isolation of the walnut pathogen Brenneria rubrifaciens, causal agent of deep bark canker. European Journal of Plant Pathology, 122, 413-424.

 

Moragrega, C., Matias, J., Aleta, N., Montesinos, E., & Rovira, M. (2011). Apical necrosis and premature drop of Persian (English) walnut fruit caused by Xanthomonas arboricola pv. juglandis. Plant Disease, 95, 1565-1570.

 

Morone C., Janse, J.D., & Scortichini, M. (1998). Bark canker of Persian walnut (Juglans regia) tree incited by Erwinia nigrifluens in Italy. Journal of Phytopathology, 146, 637-639.

 

Ozaktan, H., Akat, S., Akkopru, A., & Yolageldi, L. (2015). Updating the information on disease problems of walnut cultivation in Turkey. In 1st European Fruit Research Institutes Network Shell Fruit Species Meeting. Budapest, Hungary: NARIC Fruitculture Research Institute.

 

Popović, T., Ivanović, Ž., Živković, S., Trkulja, N., & Ignjatov, M. (2013). First report of Brenneria nigrifluens as the causal agent of shallow-bark canker on walnut trees (Juglans regia) in Serbia. Plant Disease, 97(11), 1504.

 

Schaad N.W., Jones J.B., & Chun W., eds. (2001). Laboratory guide for identification of plant pathogenic bacteria (3rd edition). St. Paul, MN: APS Press.

 

Temperini, C.V., Pardo, A.G., Pose, G.N. (2017). First report of apical necrosis in walnut cultivars from Northern Argentinean Patagonia. Journal of Plant Pathology and Microbiology, 8(7), 1-6.

Objavljeno
2021/12/20
Kako citirati
Iličić, R., Bagi, F., Blagojević, M., Gošić, J., Milovanović, P., & Popović, T. (2021). Etiologija bakterioznih oboljenja mladih zasada oraha u Srbiji. Pesticides and Phytomedicine / Pesticidi I Fitomedicina, 36(3), 101-109. https://doi.org/10.2298/PIF2103101I
Rubrika
Originalni naučni članak