Effect of neuropeptide Y on norepinephrine-induced constriction in the rabbit facial artery after carotid artery occlusion
Abstract
Background/Aim. Atherosclerotic-occlusive changes could be observed in orofacial branches of the external carotid artery. Atherosclerosis-induced ischemia caused alteration in production and release of endothelial factors. The aim of this study was to investigate the influence of carotid artery occlusion (10, 30 and 60 min) on vascular effects of norepinephrine (NOR) and neuropeptide Y (NPY) in the isolated glandular branch of the rabbit facial artery, the main feeding artery for the submandibular gland. Method. Changes in isometric tension were recorded in organ bath studies with arterial rings, before and after carotid artery occlusion. Results. Concentration-dependent vasocontractile effect of NOR was significantly augmented after 30 and 60 min of carotid occlusion, but only in the rings with intact endothelium. Given alone, NPY showed no effect in isolated glandular branch of the rabbit facial artery, but enhanced NOR vasoconstriction in all the investigated rings. NOR vasocontractile effect enhancement in the presence of NPY was attenuated after 30 and 60 min of carotid occlusion. Also, enhancement of NOR vasoconstriction by NPY was significantly higher in endothelium-intact rings compared to endothelium-denuded rings obtained after 30 and 60 min of carotid occlusion. Conclusion. The present investigation provides results of increased vasocontractile effect of NOR and decreased enhancing effect of NPY on NOR vasoconstriction in the rabbit facial artery after carotid occlusion that is related to altered endothelium function.
References
Edwards AV. Autonomic control of salivary blood flow. In: Garrett JR, Ekström J, Anderson LC, editors. Glandular mechan-isms of salivary secretion. Frontiers of oral biology. Basel: Karger; 1998. p. 110−7.
Lohman AW, Billaud M, Isakson BE. Mechanisms of ATP release and signalling in the blood vessel wall. Cardiovasc Res 2012; 95(3): 269−80.
Hirsch D, Zukowska Z. NPY and stress 30 years later: the peri-pheral view. Cell Mol Neurobiol 2012; 32(5): 645−59.
Ekström J, Ekman R, Luts A, Sundler F, Tobin G. Neuropeptide Y in salivary glands of the rat: origin, release and secretory ef-fects. Regul Peptides 1996; 61(2): 125−34.
Kusakabe T, Matsuda H, Kawakami T, Syoui N, Kurihara K, Tsu-kuda M, et al. Distribution of neuropeptide-containing nerve fibers in the human submandibular gland, with special refer-ence to the difference between serous and mucous acini. Cell Tissue Res 1997; 288(1): 25−31.
Edvinsson L, Ekblad E, Håkanson R, Wahlestedt C. Neuropeptide Y potentiates the effect of various vasoconstrictor agents on rabbit blood vessels. Br J Pharmacol 1984; 83(2): 519−25.
Kwan YW, Wadsworth RM, Kane KA. Effects of neuropeptide Y and calcitonin gene-related peptide on sheep coronary artery rings under oxygenated, hypoxic and simulated myocardial ischaemic conditions. Br J Pharmacol 1990;99(4):774-8.
Hunter LW, Tyce GM, Rorie DK. Neuropeptide Y release and contractile properties: differences between canine veins and ar-teries. Eur J Pharmacol 1996; 313(1−2): 79−87.
Gullestad L, Pernow J, Bjurö T, Aaberge L, Skårdal R, Kjekshus E, et al. Differential effects of metoprolol and atenolol to neuro-peptide Y blockade in coronary artery disease. Scand Cardio-vasc J 2012; 46(1): 23−31.
Gullestad L, Jorgensen B, Bjuro T, Pernow J, Lundberg JM, Dota CD, et al. Postexercise ischemia is associated with increased neuro-peptide Y in patients with coronary artery disease. Circulation 2000; 102(9): 987−93.
Pankajakshan D, Jia G, Pipinos I, Tyndall SH, Agrawal DK. Neu-ropeptide Y receptors in carotid plaques of symptomatic and asymptomatic patients: effect of inflammatory cytokines. Exp Mol Pathol 2011; 90(3): 280−6.
Michiels C. Physiological and pathological responses to hypoxia. Am J Pathol 2004; 164(6): 1875−82.
Suarez-Cunqueiro MM, Duker J, Liebehenschel N, Schön R, Schmel-zeisen R. Calcification of the branches of the external carotid artery detected by panoramic radiography: a case report. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002; 94(5): 636−40.
Vág J, Hably C, Kerémi B, Kovács E, Bartha J, Fazekas A. Role of nitric oxide in the regulation of blood flow in the rat subman-dibular gland during carotid artery occlusion. Arch Oral Biol 2001; 46(3): 261−7.
Widlansky ME, Gokce N, Keaney JF, Vita JA. The clinical impli-cations of endothelial dysfunction. J Am Coll Cardiol 2003; 42(7): 1149−60.
Ruijtenbeek K, Kessels CG, Villamor E, Blanco CE, De Mey JG. Di-rect effects of acute hypoxia on the reactivity of peripheral ar-teries of the chicken embryo. Am J Physiol Regul Integr Comp Physiol 2002; 283(2): R331−8.
Juránek I, Bauer V, Donnerer J, Lembeck F, Peskar BA. Severe hy-poxia inhibits prostaglandin I(2) biosynthesis and vasodilatory responses induced by ionophore A23187 in the isolated rabbit ear. Pharmacology 2002; 66(4): 199−205.
Sobey CG. Potassium channel function in vascular disease. Ar-terioscler Thromb Vasc Biol 2001; 21(1): 28−38.
Roganović J, Radenković M, Stojić D. ACh- and VIP-induced vaso-relaxation in rabbit facial artery after carotid artery occlusion. Arch Oral Biol 2010; 55(5): 333−42.
Pagán RM, Martínez AC, Hernández M, Martínez MP, García-Sacristán A, Correa C, et al. Endothelial and neural factors func-tionally involved in the modulation of noradrenergic vasocon-striction in healthy pig internal mammary artery. Biochem Pharmacol 2012; 83(7): 882−92.
Xavier FE, Blanco-Rivero J, Avendaño MS, Sastre E, Yela R, Veláz-quez K, et al. Aldosterone alters the participation of endothelial factors in noradrenaline vasoconstriction differently in resis-tance arteries from normotensive and hypertensive rats. Eur J Pharmacol 2011; 654(3): 280−8.
Durand MJ, Gutterman DD. Diversity in mechanisms of endo-thelium-dependent vasodilation in health and disease. Micro-circulation 2013; 20(3): 239−47.
Cortés V, Donoso MV, Brown N, Fanjul R, López C, Fournier A, et al. Synergism between neuropeptide Y and norepinephrine highlights sympathetic cotransmission: studies in rat arterial mesenteric bed with neuropeptide Y, analogs, and BIBP 3226. J Pharmacol Exp Ther 1999; 289(3): 1313−22.
Hieble JP, Duesler JG, Daly RN. Effects of neuropeptide Y on the response of isolated blood vessels to norepinephrine and sympathetic field stimulation. J Pharmacol Exp Ther 1989; 250(2): 523−8.
Lewis CJ, Evans RJ, Neild TO. Inhibition of vasoconstriction and Ca2+ currents mediated by neuropeptide Y Y2 receptors. J Smooth Muscle Res 1999; 35(5−6): 147−56.
Abdel-Samad D, Jacques D, Perreault C, Provost C. NPY regulates human endocardial endothelial cell function. Peptides 2007; 28(2): 281−7.
Zukowska-Grojec Z, Karwatowska-Prokopczuk E, Rose W, Rone J, Movafagh S, Ji H, et al. Neuropeptide Y: a novel angiogenic fac-tor from the sympathetic nerves and endothelium. Circ Res 1998; 83(2): 187−95.