Efekat neuropeptida Y na konstrikciju facijalne arterije kunića izazvane norepinefrinom posle okluzije karotidne arterije

Jelena Roganović; Nina Petrović; Ljiljana Djukić

doi:10.2298/3524

Jelena Roganović Department of Pharmacology in Dentistry, Faculty of Dental Medicine, University of Belgrade, Serbia
Nina Petrović Department of Radiobiology and Molecular Genetics, Institute of Nuclear Sciences “Vinča”, University of Belgrade, Belgrade, Serbia
Ljiljana Djukić Department of Pharmacology in Dentistry, Faculty of Dental Medicine, University of Belgrade, Serbia

DOI: https://doi.org/10.2298/3524

Ključne reči: neuropeptides||, ||neuropeptidi, norepinephrine||, ||noradrenalin, carotid arteries||, ||aa. carotis, carotid stenosis||, ||okluzija, rabbits||, ||zečevi, vasoconstriction||, ||vazokonstrikcija,

Sažetak

Uvod/Cilj. Pokazano je da se okluzivne promene aterosklerotične prirode mogu opaziti na orofacijalnim granama spoljne karotidne arterije. Ishemija izazvana aterosklerozom, dovodi do poremećaja stvaranja i oslobađanja faktora poreklom iz endotela. Cilj ovog istraživanja bio je da se ispita uticaj okluzije karotidne arterije (10, 30 i 60 min) na vazokontraktilne efekte norepinefrina (NOR) i neuropeptida Y (NPY) na izolovanoj žlezdanoj grani facijalne arterije kunića, glavne dovodne arterije za submandibularnu žlezdu. Metode. U kupatilu za izolovane krvne sudove ispitivane su izometrijske promene tonusa arterijskih preparata, pre i posle okluzije karotidne arterije. Rezultati. Koncentracijski-zavisan vazokontraktilni efekat NOR bio je značajno veći posle 30 i 60 min karotidne okluzije, ali samo na preparatima sa očuvanim endotelom. Primenjen u rastućim koncentracijama, NPY nije imao efekta na tonus izolovane žlezdane grane facijalne aterije kunića, ali je povećao vazokontraktilni efekat NOR na svim ispitivanim preparatima. Povećanje vazokontraktilnog efekta NOR u prisustvu NPY bilo je značajno manje posle 30 i 60 min okluzije. Takođe, efekat NPY posle karotidne okluzije bio je značajno veći na preparatima sa očuvanim endotelom u odnosu na preparate sa uklonjenim endotelom. Zaključak. Ovo istraživanje pokazalo je povećanje vazokontraktilnog efekta NOR i smanjenje potencirajućeg efekta NPY na vazokonstrikciju izazvanu NOR na facijalnoj arteriji kunića posle okluzije karotidne arterije koje su povezane sa promenjenom funkcijom endotela.

Biografije autora

Jelena Roganović, Department of Pharmacology in Dentistry, Faculty of Dental Medicine, University of Belgrade, Serbia

Asistent, Katedra za Stomatološku farmakologiju

Nina Petrović, Department of Radiobiology and Molecular Genetics, Institute of Nuclear Sciences “Vinča”, University of Belgrade, Belgrade, Serbia

Istraživač-saradnik, Laboratorija za radiobiologiju i molekularnu genetiku

Ljiljana Djukić, Department of Pharmacology in Dentistry, Faculty of Dental Medicine, University of Belgrade, Serbia

Asistent, Katedra za Stomatološku farmakologiju

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