Mikroskopska i spektroskopska karakterizacija orašica i sluzi Ocimum basilicum i Thymus vulgaris

  • Ana Alimpić Aradski
  • Ilinka Pećinar Univerzitet u Beogradu, Poljoprivredni fakultet, Katedra za agrobotaniku, Nemanjina 6, 11000 Beograd, Srbija
  • Smiljana Janković Univerzitet u Beogradu, Biološki fakultet, Institut za botaniku i Botanička bašta "Jevremovac", Studentski trg 16, 11000 Beograd, Srbija
  • Petar D. Marin Univerzitet u Beogradu, Biološki fakultet, Institut za botaniku i Botanička bašta "Jevremovac", Studentski trg 16, 11000 Beograd, Srbija
  • Dejan Pljevljakušić Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Tadeuša Košćuška 1, 11000 Beograd, Srbija
  • Zora Dajić-Stevanović Univerzitet u Beogradu, Poljoprivredni fakultet, Katedra za agrobotaniku, Nemanjina 6, 11000 Beograd, Srbija
  • Katarina Šavikin Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Tadeuša Košćuška 1, 11000 Beograd, Srbija
  • Sonja Duletić-Lauševič Univerzitet u Beogradu, Biološki fakultet, Institut za botaniku i Botanička bašta "Jevremovac", Studentski trg 16, 11000 Beograd, Srbija
Ključne reči: Thymus, Ocimum, Lamiaceae, orašice, sluz, mikromorfologija, spektroskopija

Sažetak


Ocimum basilicum L. (bosiljak) i Thymus vulgaris L. (timijan) su dobro poznate lekovite biljke familije Lamiaceae. Iako su mikromorfološke karakteristike orašica i produkcija njihove sluzi do sada delimično proučeni, cilj ovog rada je bio analiza uzoraka iz Srbije. Rezultati dobijeni steremikroskopom i skenirajućom elektronskom mikroskopijom ukazuju da se orašice proučavanih vrsta razlikuju po obliku, veličini, stopalnom ožiljku i obrascu ornamentacije. Iako su obe vrste produkovale mlečno belu sluz sa fibrilima, orašice bosiljka su se izdvojile po brzoj reakciji osluznjavanja, kao i produkciji veće količine sluzi. Takođe, ovo je prvi izveštaj o hemijskom sastavu orašica i sluzi dve vrste familije Lamiaceae koje su gajene u Srbiji i analizirane Ramanovom spektroskopijom. Evidentirane su pojedine razlike u hemijskom profilu orašica i sluzi među analiziranim vrstama. Razlike u sastavu orašica su uglavnom pripisane sadržaju fenolnih komponenti, masnih kiselina i proteina. Proučavane orašice predstavljaju bogat izvor fenola, nezasićenih masnih kiselina i polisaharida. Raman spektroskopija se pokazala kao veoma pogodna tehnika za analizu ovog tipa uzoraka, s obzirom da omogućava identifikaciju i ukazuje na prostorni raspored biljnih metabolita u uzorku. 

Reference

Abbas, A. J., Mousa, M. A., Al-aubadi, I. K.: Chemical composition of thyme seeds Thymus vulgaris and its antimicrobial activity. Anbar Journal of Agricultural Sciences, 9(2), 2011.


Angers, P., Morales, M. R., Simon,  J. E.: Fatty acid variation in seed oil among Ocimum Species. American Oil Chemists' Society, 73(3), 393-395, 1996.


Anjaneyalu, Y. V., Gowda, D. C.: Structural studies of an acidic polysaccharide from Ocimum basilicum seeds. Carbohydrate Research, 75, 251-256, 1979.


Azuma, J. I., Sakamoto, M.: Cellulosic hydrocolloid system present in seed of plants. Trends in Glycoscience and Glycotechnology, 15(81), 1-14, 2003.


Baeten, V., Dardenne, P., Aparicio, R.: Interpretation of fourier transform raman spectra of the unsaponifiable matter in a selection of edible oils. Journal of Agricultural and Food Chemistry, 49, 5098–5107, 2001.


Baranska, M., Schulz, H., Baranski, R., Nothnagel, T., Christensen, L. P.: In situ simultaneous analysis of polyacetylenes, carotenoids and polysaccharides in carrot roots. Journal of Agricultural and Food Chemistry, 53(17), 6565-6571, 2005.


Baranski, R., Baranska, M., Schulz, H., Simon, P. W., Nothnagel, T.: Single seed raman measurements allow taxonomical discrimination of Apiaceae accessions collected in gene banks. Biopolymers, 81(6), 497-505, 2006.


Calderón Bravo, H., Vera Céspedes, N., Zura-Bravo, L., Muñoz, L. A.: Basil seeds as a novel food, source of nutrients and functional ingredients with beneficial properties: a review. Foods, 10(7), 1467, 2021.


Chylińska, M., Szymańska-Chargot, M., Zdunek, A.: Imaging of polysaccharides in the tomato cell wall with Raman microspectroscopy. Plant Methods, 10(1), 1-9, 2014.


Clopton, R. E.: Standard nomenclature and metrics of plane shapes for use in gregarine taxonomy. Comparative Parasitology, 71(2), 130-140, 2004.


Czamara, K., Majzner, K., Pacia, M. Z., Kochan, K., Kaczor, A., Baranska, M.: Raman spectroscopy of lipids: a review. Journal of Raman Spectroscopy, 46(1), 4-20, 2014.


De Gelder, J., De Gussem, K., Vandenabeele, P., Moens, L.: Reference database of Raman spectra of biological molecules. Journal of Raman spectroscopy, 38, 1133–1147, 2007.


Diklić, N.: Flora SR Srbije, Vol VI: SANU, Beograd, 1974.


Duletic-Lausevic, S., Marin P.D.: Pericarp structure and myxocarpy in
selected genera of Nepetoideae (Lamiaceae). Nordic Journal of Botany, 19(4),
435–446, 1999.


Fei, G., Lujia, H., Xian, L.: Vibration spectroscopic technique for species identification based on lipid characteristics. International Journal of Agricultural and Biological Engineering, 10(3), 255-268, 2017.


Fernández-Alonso, J. L., Vega, N., Filgueira, J. J., Pérez, G.: Lectin prospecting in Colombian Labiatae. A systematic-ecological approach. Biochemical Systematics and Ecology, 31(6), 617-633, 2003.


Himmelsbach, D.S., Akin, D.E.: Near-infrared–Fourier-transform–Raman spectroscopy of flax (Linum usitatissimum L.) stems. Journal of Agricultural and Food Chemistry, 46, 991-998, 1998.


Hosseini-Parvar, S.H., Matia-Merino, L., Goh, K.K.T., Razavi, S.M.A., Mortazavi, S.A.: Steady shear flow behavior of gum extracted from Ocimum basilicum L. seed: Effect of concentration and temperature. Journal of Food Engineering, 101, 236–243, 2010.


Jain, N., Choudhary, P.: Phytochemistry, traditional uses and pharmacological aspect of Thymus vulgaris: a review. Indian Journal of Pharmaceutical Sciences, 84(6), 1369-1379, 2022.


Kreitschitz, A., Gorb, S. N.: The micro-and nanoscale spatial architecture of the seed mucilage - Comparative study of selected plant species. PloS one, 13(7), e0200522, 2018.


Li-Chan, E. C. Y.: The applications of Raman spectroscopy in food science. Trends in Food Science and Technology, 7, 361–370, 1996a.


Lv, M. Y., Zhang, X., Ren, H. R., Liu, L., Zhao, Y. M., Wang, Z. et al.: A rapid method to authenticate vegetable oils through surface-enhanced Raman scattering. Scientific Reports, 6, 23405, 2016.


Malekfar, R., Nikbakht, A. M., Abbasian, S., Sadeghiand, F., Mozaffari, M.: Evaluation of tomato juice quality using surface enhanced Raman spectroscopy. Acta Physica Polonica A, 117(6), 971-973, 2010.


Marin, P. D., Sajdl, V., Kapor, S., Tatic, B., Petkovic, B.: Fatty acids of the Saturejoideae, Ajugoideae and Scutellarioideae (Lamiaceae). Phytochemistry, 30(9), 2979-2982, 1991.


Marin, P.D.: Nutlets and trichomes in the Lamiaceae family.
University of Belgrade, Faculty of Belgrade, Belgrade, 1996.


Martini, W. S., Porto, B. L. S., de Oliveira M. A. L., Sant’Ana, A. C.: Comparative study of the lipid profiles of oils from kernels of peanut, babassu, coconut, castor and grape by GC-FID and Raman spectroscopy.  Journal of Brazilian Chemical Society, 29(2), 390-397, 2018.


Moon, H. K., Hong, S. P., Smets, E., Huysmans, S.: Micromorphology and character evolution of nutlets in tribe Mentheae (Nepetoideae, Lamiaceae). Systematic botany, 34(4), 760-776, 2009.


Muik, B., Lendl, B., Molina-Díaz, A., Ayora-Cañada M.J..: Direct monitoring of lipid oxidation in edible oils by Fourier transform Raman spectroscopy. Chemistry and physics of lipids, 134(2), 173-82, 2005.


Nazir, S., Wani, I. A., Masoodi, F. A.: Extraction optimization of mucilage from Basil (Ocimum basilicum L.) seeds using response surface methodology. Journal of Advanced Research, 8(3), 235-244, 2017.


Purushothaman, B., Prasanna Srinivasan, R., Suganthi, P., Ranganathan, B., Gimbun, J., Shanmugam, K.: A comprehensive review on Ocimum basilicum. Journal of Natural Remedies, 71-85, 2018.


Razavi, S. M., Mortazavi, S. A., Matia-Merino, L. Hosseini-Parvar, S. H., Motamedzadegan, A., Khanipour, E.: Optimisation study of gum extraction from Basil seeds (Ocimum basilicum L.), International Journal of Food Science and Technology, 44, 1755–1762, 2009.


Ryding, O.: Pericarp structure and phylogeny of tribe Mentheae (Lamiaceae). Plant systematics and evolution, 285, 165-175, 2010.


Ryding, O.: Pericarp structure and phylogeny within Lamiaceae subfamily Nepetoideae tribe Ocimeae. Nordic Journal of Botany, 12(3), 273-298, 1992.


Salgado-Cruz, M. D. L. P., Calderón-Domínguez, G., Chanona-Pérez, J., Farrera-Rebollo, R. R., Méndez-Méndez, J. V., Díaz-Ramírez, M.: Chia (Salvia hispanica L.) seed mucilage release characterisation. A microstructural and image analysis study. Industrial Crops and Products, 51, 453-462, 2013.


Schulz, H., Baranska, M.: Identification and quantification of valuable plant substances by IR and Raman spectroscopy. Vibrational Spectroscopy, 43(1), 13-25, 2007.


Strehle, M. A., Rösch, P., Baranska, M., Schulz, H., Popp J.: On the way to a quality control of the essential oil of fennel by means of Raman spectroscopy. Biopolymers, 77(1), 44–52, 2005.


Synytsya, A., Copikova, J., Matejka, P., Machovic, V.: Fourier transform Raman and infrared spectroscopy of pectins. Carbohydrate polymers, 54, 97–106, 2003.


Thygesen, L. G., Løkke, M. M., Micklander, E., Engelsen, S. B.: Vibrational microspectroscopy of food. Raman vs. FT-IR. Trends in Food Science & Technology, 14(1-2), 50-57, 2003.


Tobolova, M., Kresalek, V., Vaškova, H., Bučkova, M.: Investigation of the thermal degradation of vegetable oils by spectroscopic methods and its mathematical analysis. International Journal of Mathematics and Computers in Simulations, 8(1), 213-219, 2014.


Vaškova, H., Bučkova, M.: Measuring and identification of oils. In Proceedings of the 18th International Conference on Systems (part of CSCC´14), Santorini Island, 211–215, 2014.


Vaškova, H., Bučkova, M.: Thermal degradation of vegetable oils: spectroscopic measurement and analysis. Procedia Engineering, 100, 630-635, 2015.


Weng, Y. M., Weng, R. H., Tzeng, C. Y., Chen, W.: Structural analysis of triacylglycerols and edible oils by near-infrared Fourier transform Raman spectroscopy. Applied spectroscopy, 57(4), 413-418, 2003.


 


Zhao, F., Chen, Y.P., Salmaki, Y., Drew, B. T., Wilson, T.C., Scheen, A. C. et al..: An updated tribal classification of Lamiaceae based on plastome phylogenomics. BMC Biology, 19, 1-27, 2021.

Objavljeno
2023/08/15
Broj časopisa
Vol. 32 Br. 1 (2023): Acta herbologica
Rubrika
Članci
Autori koji objavljuju u ovom časopisu pristaju na sledeće uslove:

  1. Autori zadržavaju autorska prava i pružaju časopisu pravo prvog objavljivanja rada i licenciraju ga "Creative Commons Attribution licencom" koja omogućava drugima da dele rad, uz uslov navođenja autorstva i izvornog objavljivanja u ovom časopisu.

  2. Autori mogu izraditi zasebne, ugovorne aranžmane za neekskluzivnu distribuciju članka objavljenog u časopisu (npr. postavljanje u institucionalni repozitorijum ili objavljivanje u knjizi), uz navođenje da je članak izvorno objavljen u ovom časopisu.

  3. Autorima je dozvoljeno i podstiču se da postave objavljeni članak onlajn (npr. u institucionalni repozitorijum ili na svoju internet stranicu) pre ili tokom postupka prijave rukopisa, s obzirom da takav postupak može voditi produktivnoj razmeni ideja i ranijoj i većoj citiranosti objavljenog članka (Vidi Efekti otvorenog pristupa).