ISOLATION AND CHARACTERIZATION OF BACTERIA AND YEASTS FROM CONTAMINATED SOIL

  • Vera M Karlicic Univerzitet u Beogradu, Poljoprivredni fakultet,Katedra za ekološku mikrobiologiju
  • Danka S Radic
  • Jelena P. Jovicic Petrovic
  • Blazo T. Lalevic
  • Ljubinko M. Jovanovic
  • Dragan D. Kikovic
  • Vera B. Raicevic

Sažetak


Plant growth promoting (PGP) bacteria and yeasts play an important role in bioremediation processes. Thirty bacterial and ten yeasts isolates were obtained from PAH and PCB contaminated soil with an aim of determining the presence of PGP mechanisms (production of ammonia, indole acetic acid, siderophores and solubilization of inorganic phosphate). As a result three bacterial (Serratia liquefaciens, Micrococcus sp. and Serratia sp.) and two yeasts isolates (Candida utilis and Candida tropicalis) stood up as PGP strains. Among them Serratia sp. showed the highest indole production (25.5 µg/ml). Analyses of metal tolerance (Cu+2, Cr+6 and Ni+2) revealed that Serratia liquefaciens, Micrococcus sp., Serratia sp. and Candida tropicalis were capable to tolerate significant concentration of metals. As a results of this study several bacterial and yeast strains were attributed as potential plant growth promoters which can be applied in future remediation activities and environmental quality improvements.

Reference

Anderson, G.R. (1958): Ecology of azotobacter in soils of the Palouse region: I. Occurrence. Soil Science 86: 57-62.

Bajić, B., Rončević, Z., Puškaš, V., Miljić, U., Dodić, S., Grahovac, J., Dodić, J. (2015): White wine production effluents used for biotechnological production of xanthan. Journal on Processing and Energy in Agriculture (former PTEP) 19: 52-55.

Behera, B.C., Singdevsachan, S.K., Mishra, R.R., Dutta, S.K., Thatoi, H.N. (2013): Diversity mechanism and biotechnology of phosphate solubilising microorganism in mangrove- A review. Biocatalysis and Agricultural Biotechnology 3: 97-110.

Berg, G. (2009): Plant-microbe interactions promoting plant growth and health: perspectives for controlled use of microorganisms in agriculture. Applied Microbiology and Biotechnology 84: 11-8.

Berraquero, F.R., Baya, A.M., Cormenzana, A.R. (1976): Establishment of indices for the study of phosphate solubilization by soil bacteria. Ars Pharmacéutica 17: 399-406.

Braud, A., Jezequel, K., Bazot, S., Lebeau, T. (2009): Enhanced phytoextraction of an agricultural Cr-Hg and Pb-contaminated soil by bioaugmentation with siderophore producing bacteria. Chemosphere 74: 280-286.

Breierova, E., Certik, M., Kovarova, A., Gregor, T. (2008): Biosorption of nickel by yeasts in an osmotically unsuitable environment. Zeitschrift für Naturforschung C 63: 873-878.

Campos, V., Moraga, R., Fernandez, I., Yanez, F., Valenzuela, A., Mondac, M. A. (2013): Reduction of hexavalent cromium by Serratia marcecens immobilized on active carbon and their potencial use in bioremediation. Gayana 77: 60-63.

Cappuccino, J.C., Sherman, N. (1992): In: Microbiology: A Laboratory Manual, third ed. Benjamin/cummings Pub. Co. New York.

Christry, M., Ramaligam, R. (2005): Vermicomposting of sago industrial solid waste using epigeic earthworm Eudrilus eugeniae and macronutrients analysis of vermicompost. Asian Journal of Microbiology Biotechnology and Environmental Sciences 7: 377-381.

Compant, S., Duffy, B., Nowak, J., Clement, C., Barka, E.A. (2005): Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. Applied Environmental Microbiology 71: 4951–4959.

Glick, B.R. (2012): Plant Growth-Promoting Bacteria: Mechanisms and Applications. Hindawi Publishing Corporation Scientifica: Article ID 963401, http://dx.doi.org/10.6064/2012/963401.

Hayat, R., Ali, S., Amara, U., Khalid, Rabia, Ahmed, I. (2010): Soil beneficial bacteria and their role in plant growth promotion: a review. Annals of Microbiology 60: 579–598.

Huang, X.D., El-Alawi, Y., Gurska, Jolanta, Glick, B.R., Greenberg, B.M. (2005): A multi-process phytoremediation system for decontamination of persistent total petroleum hydrocarbons (TPHs) from soil. Microchem Journal 8: 139-47.

Jovičić Petrović, J., Karličić, V., Radić, D., Jovanović, Lj., kiković, D., Raičević, V. (2014): Microbial Biodiversity in PAH and PCB Contaminated Soil as a Potential for in Situ Bioremediation. Proceedings of The 9th Conference on Sustainable Development of Energy, Water and Environment Systems. Venice/Istanbul, Italy/ Turkey, pp.1-10.

Kavamura, V.N., Esposito, E. (2010): Biotechnological strategies applied to the decontamination of soils polluted with heavy metals. Biotechnology Advances 28: 61-69.

Karličić, V., Jovičić Petrović, J., Radić, D., Lalević, B., Raičević, V., Jovanović. Lj. (2014): In situ bioremediation of soil polluted with organotin substrances. In: Vrvić, M., Cokić, Z., Tanasijević, Lj. (Edd.), Proceedings of the „Soil 2014“ Planning and land use and landfills in terms of sustainable development and new remediation technologies. Zrenjanin , Serbia, pp. 43-50.

Koo, S.Y., Cho, K.S. (2009): Isolation and characterization of a plant growth promoting rhizobacterium Serratia sp. SY5. Journal of Microbiology and Biotechnology 19: 1431–1438.

Lucy, M., Reed, E., Glick, B.R. (2004): Applications of free living plant growth-promoting rhizobacteria. Antonie van Leeuwenhoek 86: 1-25.

Ma, Y., Prasad, M.N.V., Rajkumar, M., Freitas, H. (2011): Plant growth promoting rhizobacteria and endophytes accelerate phytoremediation of metalliferous soil. Biotechnology Advances 29: 248-58.

Mirabal, A.L., Kleiner, D., Ortega, E. (2008): Spores of the mycorrhizal fungus Glomus mosseae host yeasts that solubilize phosphate and accumulate polyphosphates. Mycorrhiza 18: 197–204.

Patten C.L., Glick, B.R. (2002): Role f Pseudomonas putida Indoleacetic Acid in Development of the Host Plant Root System. Applied and Environmental Microbiology 68: 3759-3801.

Rodrigez, H., Fraga, R., Gonzalez, T., Bashan, Y. (2006): Genetics of phosphate solubilization and its potential applications for improving plant growth-promoting bacteria. Plant and Soil 287: 15–21.

Schwyn, B., Neilands, J.B. (1987): Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry 160: 47–56.

Sarma, B., Acharya, C., Joshi, S. R. (2013): Characterization of Metal Tolerant Serratia spp. Isolates from Sediments of Uranium Ore Deposit of Domiasiat in Northeast India. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. doi 10.1007/s40011-013-0236-0

Smith, S., Read, D. (2008): Mycorrhizal Symbiosis, 3rd ed. Academic Press, San Diego, CA, USA.

Teixeira, D., Alfenas, A., Goncalves, R.M., Mafia, G. R., Siqueira, L., Maffia, L., Mounteer, A. (2007): Rizobacterial promotion of eucalypt rooting and growth. Brazilian Journal of Microbiology 38: 118-123.

Tilak, K.V.B.R., Ranganayaki, N., Pal, K.K., De, R., Saxena, A.K., Nautiyal, S.C., Mittal, S., Tripathi, A.K., Johri, B.N. (2005): Diversity of plant growth and soil health supporting bacteria. Current science 89.

Trama, B., Santos Fernandes, J. D., Labuto, G., Oliveira, J. C. F., Viana-Niero, C., Pascon, R.C., Vallim, M.A. (2014): The Evaluation of Bioremediation Potential of a Yeast Collection Isolated from Composting. Advances in Microbiology 4: 796-807.

Welbaum, G., Sturz, A.V., Dong, Z., Nowak, J. (2004): Fertilizing soil microorganisms to improve productivity of agroecosystems. Critical Reviews in Plant Sciences 23: 175-93.

Objavljeno
2016/10/17
Rubrika
Originalni naučni članak