Effects of avocado seed extract in different tris-extenders on sperm and oxidative stress parameters of West African Dwarf goat spermatozoa during vitrification

James Ola Daramola; Oluwakemi Dayo Onanuga; Monsuru Oladimeji Abioja; Mathew Adeleke; Olajide Olowofeso; Oyegunle Emmanuel Oke; Ezekiel Oluwafemi Adekunle; Taiwo Amidu Sorongbe; Olayinka Ayobami Iyanda

James Ola Daramola Department of Animal Physiology, Federal University of Agriculture Abeokuta, P.M.B 2240, 110001 Abeokuta, Ogun State, Nigeria.
Oluwakemi Dayo Onanuga Department of Animal Physiology, Federal University of Agriculture Abeokuta, Nigeria
Monsuru Oladimeji Abioja Department of Animal Physiology, Federal University of Agriculture Abeokuta, Nigeria
Mathew Adeleke Animal Breeding & Genetics Department, Federal University of Agriculture Abeokuta, Nigeria
Olajide Olowofeso Animal Breeding & Genetics Department, Federal University of Agriculture Abeokuta, Nigeria
Oyegunle Emmanuel Oke Department of Animal Physiology, Federal University of Agriculture Abeokuta, Nigeria
Ezekiel Oluwafemi Adekunle Department of Animal Physiology, Federal University of Agriculture Abeokuta, Nigeria
Taiwo Amidu Sorongbe Department of Animal Physiology, Federal University of Agriculture Abeokuta, Nigeria
Olayinka Ayobami Iyanda Department of Animal Physiology, Federal University of Agriculture Abeokuta, Nigeria

Keywords: sperm, Seed extracts, extracts, extender, Avocados,

Abstract

Abstract: Effects of avocado seed extract (ASE) in different tris-extenders on sperm and oxidative stress parameters of West African Dwarf (WAD) goat during vitrification were evaluated. Semen samples were diluted with different levels of ASE (0, 5, 10, 15 and 20 mL/100 mL of diluent) each in tris citric acid (TCA), tris sodium phosphate (TSP) and tris sodium citrate (TSC) extenders while Tris-extender without citric acid, sodium phosphate and sodium citrate served as control. Diluted semen samples were vitrified and stored for 30 days. The results showed higher (P<0.05) sperm motility in TCA extenders at 10 % 15 % and 20 %, and TSP extender at 20 % ASE. Higher (P<0.05) acrosome integrities were observed in the different tris-extenders at 15 % and 20 % ASE. TCA, TSP and TSC extenders had higher (P<0.05) membrane integrities at 10 %, 15 % and 20 % ASE. TSP extenders had higher (P<0.05) live sperm at 20 % ASE. TCA extenders had higher acrosome reaction while TSP at 20% ASE and TSC at 15% and 20% of ASE had higher sperm capacitation. TCA extender at 20% ASE had reduced malondialdehyde (MDA) concentrations while higher acrosin activities were observed at 20% ASE in TCA extender (P<0.05). The findings indicated that 20% ASE in the tris-extenders improved sperm and oxidative stress parameters and the tris-extenders is ranked best in this order: TCA > TSP > TSC.

Author Biographies

James Ola Daramola, Department of Animal Physiology, Federal University of Agriculture Abeokuta, P.M.B 2240, 110001 Abeokuta, Ogun State, Nigeria.

Dept of Animal Physiology, College of Animal Science, Federal University of AgricultureAbeokuta, Nigeria.

Associate Professor

Monsuru Oladimeji Abioja, Department of Animal Physiology, Federal University of Agriculture Abeokuta, Nigeria

PhD

Mathew Adeleke, Animal Breeding & Genetics Department, Federal University of Agriculture Abeokuta, Nigeria

PhD

Olajide Olowofeso, Animal Breeding & Genetics Department, Federal University of Agriculture Abeokuta, Nigeria

Associate Professor

Oyegunle Emmanuel Oke, Department of Animal Physiology, Federal University of Agriculture Abeokuta, Nigeria

PhD

Ezekiel Oluwafemi Adekunle, Department of Animal Physiology, Federal University of Agriculture Abeokuta, Nigeria

PhD

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