ANESTHETIC EFFECT OF CLOVE (Eugenia aromaticum) SEED EXTRACT ON Clarias gariepinus (BURCHELL, 1822) FINGERLINGS UNDER SEMI ARID CONDITION

Sažetak

The anesthetic effects of Clove (Eugenia aromaticum) seed extract on Clarias gariepinus fingerlings were investigated under semi-arid condition. Various concentrations of the clove seed extract at 25.0, 50.0, 75.0, 100.0, 125.0 and 150.0mg/per liter of water were used for the experiment. Each concentration was tested on a group of 10 Clarias gariepinus fingerlings (24.13 - 25.30g weight and 5.97 - 7.00 cm length) in glass aquariums.  There was a decrease in induction time as the concentration of the clove seed extract increased. Fingerlings treated with 150mg/l of the extract produced shortest induction time (2.28±0.15 minutes), followed by fish treated with 100 and 125mg/l (3.31±0.55 and 3.07±0.07 minutes, respectively). Longest induction time (10.60±0.98, 7.52±0.25 and 5.96±1.17 minutes) was observed in fingerling sedated with 50, 25 and 75mg/l, respectively. Recovery was significantly faster (2 – 3.92 minutes) in fish treated with lower dosages (25 to 125mg/l) of the clove seed extract. Mortality after 24 hours of recovery were higher (4.79±0.25 and 3.10±0.54 %) in fish anaesthetized with higher (150 and 125mg/l) concentration of clove seed extract, respectively.  The clove seed extract is an effective anesthesia in Clarias fingerling, therefore, could be utilized for transportation of fingerling to reduce stress and mortality.