DEHYDROGENAZE POLYMORPHISM AS A TOOL FOR EARLY SELECTION OF LOW VIGOUR ROOTSTOCK FOR SWEET AND SOUR CHERRY

Slavica D Čolić; Milica M Fotirić Akšić; Vera S Rakonjac; Dragan T Nikolić

Slavica D Čolić Institute for Science Application in Agriculture,Blvd. despota Stefana 68b, 1000 Belgrade, Serbia
Milica M Fotirić Akšić Faculty of Agriculture, University of Belgrade, Nemanjina 6, Zemun, 11080 Belgrade, Serbia
Vera S Rakonjac Faculty of Agriculture, University of Belgrade, Nemanjina 6, Zemun, 11080 Belgrade, Serbia
Dragan T Nikolić Faculty of Agriculture, University of Belgrade, Nemanjina 6, Zemun, 11080 Belgrade, Serbia

Abstract

Correlation of dehydrogenase isoenzyme profile and plant/tree vigour of twenty genotypes belonging to genus Prunus, subgenus Cerasus, and four commercial rootstocks (Colt, Gisela 5, Gisela 6 and MaxMa 14), was investigated in this study. PCA was applied for the classification of the cherry rootstock based on vigor traits and polymorphism of isoenzymes in order to determine which dehydrogenase enzyme is the most useful in the preselecting process ofcherry rootstocks. The most influential variables which led to the separation of low vigorous from other genotypes were IDH, MDH and PGD. Since both significant and negative correlation observed between tree vigour and MDH, so as between tree vigour and PGD, those systems can be used for early selection of low vigour rootstocks. Our results suggest that association between low vigour and MDH genotype ab, provides a convenient marker that can be characterized even in the seedling stage, and could be applied in early rootstock selection and breeding programs

Author Biographies

Slavica D Čolić, Institute for Science Application in Agriculture,Blvd. despota Stefana 68b, 1000 Belgrade, Serbia

Associate Research, Genetics and breeding of fruits and grapes

Milica M Fotirić Akšić, Faculty of Agriculture, University of Belgrade, Nemanjina 6, Zemun, 11080 Belgrade, Serbia

Associate Professor, department Fruit Science and Viticulture

Vera S Rakonjac, Faculty of Agriculture, University of Belgrade, Nemanjina 6, Zemun, 11080 Belgrade, Serbia

Full Professor, department Genetics, breeding and seed production

Dragan T Nikolić, Faculty of Agriculture, University of Belgrade, Nemanjina 6, Zemun, 11080 Belgrade, Serbia

Full Professor, department Fruit Science and Viticulture

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