PROFILISANJE PROTEINA SEMENA I PROCENA RAZNOLIKOSTI LUPINUS ALBUSA (L.) IZ REGIONA AMHARA, ETIOPIJA
Sažetak
Seed protein profiling of 48 lupin accessions were evaluated to assess the genetic diversity of lupin from Amhara region, Ethiopia. A total of 30 bands were detected and used for the diversity assessment. The average number of bands, percentage of polymorphic bands and gene diversity among the analysed accessions were 16.96, 20.35 and 0.072 respectively. Diversity estimates analysed by grouping accessions based on their origin revealed that the highest diversity estimates were observed in Bahir Dar area followed by West Gojam and Agew Awi zones. Pair wise comparison of genetic distances (GD) among the analysed accessions ranged from 0.011 to 0.363. The most distantly related accessions were accession 6, collected from West Gojam and accession 28 from Bahir Dar area (GD = 0.378). Principal Coordinate Analysis (PCoA) showed absence of district group and most of the accessions were intermixed. Structure analysis revealed that the 48 lupin accessions could be assigned to three clusters. Most of the accessions from Agew Awi zone were grouped together while the rest of the accessions from different zones were grouped in the same cluster indicating that genotypes from the different zones had similar genetic background.
Reference
References
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Ainouche A.K., & Bayre, R.J. (1999). Phylogenetic relationships in lupinus (Fabaceae: papilionoideae) based on internal transcribed spacer sequences (ITS) of nuclear ribosomal DNA. American Journal of Botany, 86, 590-607.
Atnaf M., Yao, N. R, Martina, K., Dagne, K., Wagray, D., & Tesfaye, K. (2017). Molecular genetic diversity and population structure of Ethiopian white lupin landraces: Implications for breeding and conservation. PLoS ONE 12(11): e0188696.
Belski, R., Mori, T.A., Puddey, I.B., Sipsas, S., Woodman, R.J., & Ackland, T.R. (2010). Effects of lupin-enriched foods on body composition and cardiovascular disease risk factors: a 12-month randomized controlled weight loss trial. International Journal of Obesity, 35(6), 810–9.
CSA. (2018). Report on area and production of crops (Private peasant holdings, Meher season). Central Statistics Agency, Statistical bulletin 586, Addis Ababa, Ethiopia.
Duranti, M. & Morazzoni, P. (2011). Nutraceutical properties of lupin seed proteins A great potential still waiting for full exploitation. Agro Food Industry Hi-Tech, 22(1), 20–3.
Earl, D.A. & vonHoldt, B. M. (2012). STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conservation Genetics Resources, 4 (2), 359-361.
Erbas, M., Certel, M. & Uslu, M.K. (2005). Some chemical properties of white lupin seeds (Lupinus albus). Food chemistry, 89 (3), 341-345.
Evanno, G., Regnaut, S., & Goudet, J. (2005). Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology, 14, 2611–2620
Falush, D., Stephens, M., & Pritchard, J.K. (2003). Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics, 164, 1567–87.
Fufa, H., Baenziger, P.S., Beecher, B., Dweikat, I., Graybosh, R.A., & Eskridge, K.M. (2005) Comparison of phenotypic and molecular marker-based classifications of hard red winter wheat cultivars. Euphytica, 145, 133–146.
Hill, G. D. (1977). The composition and nutritive value of lupin seed. Nutrition Abstracts and Reviews, 47, 511-529.
Jansen, P.C.M. (2006). Lupinus albus L. In M. Brink & G. Belay (Eds.), Plant resources of tropical Africa, Wageningen, Netherlands.
Kurlovich, B.S. & Kartuzova, L.T. (2002). The history of lupin domestication. In B.S Kurlovich (Ed.), Lupins (Geography, classification, genetic resources, and breeding). (pp 147-164). OY International North Express. St. Petersburg, Russia-Pellosniemi, Finland.
Laemmli U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685.
Magni, C., Sessa, F., Accardo, E., Vanoni, M., Morazzoni, P. & Scarafoni, A. (2004). Conglutin gamma, a lupin seed protein, binds insulin in vitro and reduces plasma glucose levels of hyperglycemic rats. Journal of Nutritional Biochemistry, 15(11), 646–50.
Mulayim, M., Tamkoc, A. & Babaoglu, M. (2002). Sweet white lupins versus local bitter genotype: agronomic characteristics as affected by different planting densities in the Goller region of Turkey. European Journal of Agronomy, 17, 181–189.
Negisho, K., Teshome, A., & Keneni, G. (2017). Genetic diversity in Ethiopian field pea (Pisum sativum L.) germplasm collections as revealed by SSR markers. Ethiopian Journal of Agricultural Science, 27(3), 33-47.
Nigussie, Z. (2012). Contribution of white lupin (Lupinus albus L.) for food security in North-Western Ethiopia: A Review. Asian Journal of Plant Sciences, 11, 200–205.
Oumer, A., Petros, Y., Tesfaye, K., Teshome, A., & Bekele, E. (2015). Inter simple sequence repeat (ISSR) analysis of Ethiopian white lupine (Lupinus albus L.). African Journal of Biotechnology, 14(18), 1552-1563.
Peakall, R., & Smouse, P.E. (2012). GenAlex 6.5. Genetic analysis in Excel. Population genetic software for teaching and research-un update. Bioinformatics, 28, 2537-2539.
Pollard, N. J., Wrigley, C.W., Bekes, F., Aumate, A., & MacRitchie, F. (1996). Distinction between genotypes of Lupinus species by sodium dodecyl sulphate-gel electrophoresis and by capillary electrophoresis. Electrophoresis, 17, 221-223.
Raman, R., Cowley, B.R., Raman ,H. & Luckett, J.D. (2014). Analyses using SSR and DArT molecular markers reveal that Ethiopian accessions of white lupin (Lupinus albus L.) represent a unique genepool. Open Journal of Genetics, 4, 87-98.
Shewry, P.R., Napier, J.A., & Tatham, A.S. (1995). Seed Storage Proteins: Structures and Biosynthesis. The Plant Cell, 7, 945-956.
Tekalign, A., Derera, J., Sibiya, J., & Mumm, R.H. (2019). Molecular analysis for genetic diversity and population structure of Ethiopian faba bean (Vicia faba L.) accessions. Journal of Plant Biology and Crop Research, 1: 1010.
Tizazu, H., & Emire S.A. (2010). Chemical composition, physicochemical and functional properties of lupin (Lupinus albus) seeds grown in Ethiopia. African Journal of Food Agriculture nutrition and development, 10(8), 3029-3046.
Vaz, A.C., Pinheiro, C., Martins, J.M.N., & Ricardo, C.P.P. (2003). Cultivar discrimination of Portuguese Lupinus albus by seed protein electrophoresis: the importance of considering ‘‘glutelins’’ and glycoproteins. Field Crops Research, 87, 23–34.
Westphal, E. (1974). Pulses in Ethiopia, their taxonomy and agricultural significance. Wageningen: Centre for Agricultural Publishing and Documentation.
Yeheyis, L., Kijora, C., Melaku, S., Girma, A., & Peters, K.J. (2010). White lupin (Lupinus albus L.), the neglected multipurpose crop: Its production and utilization in the mixed crop-livestock farming system of Ethiopia. Livestock Research for Rural Development, 22 (4). Retrieved December 16, 2020, from http://www.lrrd.org/lrrd22/4/yehe22074.htm.
Abdellatif, K.F., & Khidr, Y.A. (2010). Genetic diversity of new maize hybrids based on SSR markers as compared with other molecular and biochemical markers. Journal of Crop Science and Biotechnology, 13, 139-145.
Ainouche A.K., & Bayre, R.J. (1999). Phylogenetic relationships in lupinus (Fabaceae: papilionoideae) based on internal transcribed spacer sequences (ITS) of nuclear ribosomal DNA. American Journal of Botany, 86, 590-607.
Atnaf M., Yao, N. R, Martina, K., Dagne, K., Wagray, D., & Tesfaye, K. (2017). Molecular genetic diversity and population structure of Ethiopian white lupin landraces: Implications for breeding and conservation. PLoS ONE 12(11): e0188696.
Belski, R., Mori, T.A., Puddey, I.B., Sipsas, S., Woodman, R.J., & Ackland, T.R. (2010). Effects of lupin-enriched foods on body composition and cardiovascular disease risk factors: a 12-month randomized controlled weight loss trial. International Journal of Obesity, 35(6), 810–9.
CSA. (2018). Report on area and production of crops (Private peasant holdings, Meher season). Central Statistics Agency, Statistical bulletin 586, Addis Ababa, Ethiopia.
Duranti, M. & Morazzoni, P. (2011). Nutraceutical properties of lupin seed proteins A great potential still waiting for full exploitation. Agro Food Industry Hi-Tech, 22(1), 20–3.
Earl, D.A. & vonHoldt, B. M. (2012). STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conservation Genetics Resources, 4 (2), 359-361.
Erbas, M., Certel, M. & Uslu, M.K. (2005). Some chemical properties of white lupin seeds (Lupinus albus). Food chemistry, 89 (3), 341-345.
Evanno, G., Regnaut, S., & Goudet, J. (2005). Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology, 14, 2611–2620
Falush, D., Stephens, M., & Pritchard, J.K. (2003). Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics, 164, 1567–87.
Fufa, H., Baenziger, P.S., Beecher, B., Dweikat, I., Graybosh, R.A., & Eskridge, K.M. (2005) Comparison of phenotypic and molecular marker-based classifications of hard red winter wheat cultivars. Euphytica, 145, 133–146.
Hill, G. D. (1977). The composition and nutritive value of lupin seed. Nutrition Abstracts and Reviews, 47, 511-529.
Jansen, P.C.M. (2006). Lupinus albus L. In M. Brink & G. Belay (Eds.), Plant resources of tropical Africa, Wageningen, Netherlands.
Kurlovich, B.S. & Kartuzova, L.T. (2002). The history of lupin domestication. In B.S Kurlovich (Ed.), Lupins (Geography, classification, genetic resources, and breeding). (pp 147-164). OY International North Express. St. Petersburg, Russia-Pellosniemi, Finland.
Laemmli U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685.
Magni, C., Sessa, F., Accardo, E., Vanoni, M., Morazzoni, P. & Scarafoni, A. (2004). Conglutin gamma, a lupin seed protein, binds insulin in vitro and reduces plasma glucose levels of hyperglycemic rats. Journal of Nutritional Biochemistry, 15(11), 646–50.
Mulayim, M., Tamkoc, A. & Babaoglu, M. (2002). Sweet white lupins versus local bitter genotype: agronomic characteristics as affected by different planting densities in the Goller region of Turkey. European Journal of Agronomy, 17, 181–189.
Negisho, K., Teshome, A., & Keneni, G. (2017). Genetic diversity in Ethiopian field pea (Pisum sativum L.) germplasm collections as revealed by SSR markers. Ethiopian Journal of Agricultural Science, 27(3), 33-47.
Nigussie, Z. (2012). Contribution of white lupin (Lupinus albus L.) for food security in North-Western Ethiopia: A Review. Asian Journal of Plant Sciences, 11, 200–205.
Oumer, A., Petros, Y., Tesfaye, K., Teshome, A., & Bekele, E. (2015). Inter simple sequence repeat (ISSR) analysis of Ethiopian white lupine (Lupinus albus L.). African Journal of Biotechnology, 14(18), 1552-1563.
Peakall, R., & Smouse, P.E. (2012). GenAlex 6.5. Genetic analysis in Excel. Population genetic software for teaching and research-un update. Bioinformatics, 28, 2537-2539.
Pollard, N. J., Wrigley, C.W., Bekes, F., Aumate, A., & MacRitchie, F. (1996). Distinction between genotypes of Lupinus species by sodium dodecyl sulphate-gel electrophoresis and by capillary electrophoresis. Electrophoresis, 17, 221-223.
Raman, R., Cowley, B.R., Raman ,H. & Luckett, J.D. (2014). Analyses using SSR and DArT molecular markers reveal that Ethiopian accessions of white lupin (Lupinus albus L.) represent a unique genepool. Open Journal of Genetics, 4, 87-98.
Shewry, P.R., Napier, J.A., & Tatham, A.S. (1995). Seed Storage Proteins: Structures and Biosynthesis. The Plant Cell, 7, 945-956.
Tekalign, A., Derera, J., Sibiya, J., & Mumm, R.H. (2019). Molecular analysis for genetic diversity and population structure of Ethiopian faba bean (Vicia faba L.) accessions. Journal of Plant Biology and Crop Research, 1: 1010.
Tizazu, H., & Emire S.A. (2010). Chemical composition, physicochemical and functional properties of lupin (Lupinus albus) seeds grown in Ethiopia. African Journal of Food Agriculture nutrition and development, 10(8), 3029-3046.
Vaz, A.C., Pinheiro, C., Martins, J.M.N., & Ricardo, C.P.P. (2003). Cultivar discrimination of Portuguese Lupinus albus by seed protein electrophoresis: the importance of considering ‘‘glutelins’’ and glycoproteins. Field Crops Research, 87, 23–34.
Westphal, E. (1974). Pulses in Ethiopia, their taxonomy and agricultural significance. Wageningen: Centre for Agricultural Publishing and Documentation.
Yeheyis, L., Kijora, C., Melaku, S., Girma, A., & Peters, K.J. (2010). White lupin (Lupinus albus L.), the neglected multipurpose crop: Its production and utilization in the mixed crop-livestock farming system of Ethiopia. Livestock Research for Rural Development, 22 (4). Retrieved December 16, 2020, from http://www.lrrd.org/lrrd22/4/yehe22074.htm.
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2022/04/01
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