HAEMATOLOGY AND SERUM BIOCHEMISTRY OF PIG FED GROWER FEED FORTIFIED WITH COCOA (Theobroma cacao) SEED TESTA.
Sažetak
The on-farm attempt to cut down feed cost through bulk fortification of standard animal feed with available cheap conventional feed ingredients or wastes had been a normal practice without known empirical impact on livestock health and wellbeing. Therefore, this study determined the haematological parameters and serum biochemistry of pigs fed cocoa (Theobroma cacao) seed testa at varying inclusion levels in the formulated standard grower pig feed; 0% cocoa seed testa (T1), 25% cocoa seed testa (T2), 50% cocoa seed testa (T3), 75% cocoa seed testa (T4) and 100% cocoa seed testa (T5). Thirty (30), 8 weeks old pigs were randomly assigned to the five treatments of six pigs each and replicated thrice, with two pigs per replicate in a completely randomized design (CRD) experiment. The experiment lasted for 10 weeks. At the end of the feeding trial, blood samples were collected randomly from three pigs per treatment through the jugular vein using hypodermic needle and syringe for haematological analysis and serum biochemistry. This study recorded significant differences (P<0.05) in most of the haematological parameters, except for white blood cell (WBC), platelet and heterophils; apart from high density lipoprotein, all serum biochemical indices were also significantly different (P<0.05). Haematological parameters and serum indices were within the normal range for healthy pig except lymphocytes. However, the study revealed that haematological indices and the serum biochemical parameters indicated that cocoa seed testa at 25% level in the standard grower pig feed had the optimum support for the wellbeing and healthy performance of pigs.
Reference
Agbabiaka, L.A., Odoemenam, S.A. and Esonu, B.O. (2006). Preliminary investigation on the potentials of wild variegated cocoyam (Caladium hortulanum) replacement for maize in diets of catfish (Heterobranchus bidorsalis) fingelings. International of Agriculture and Rural Development, 7(1): 138-142.
Amefule, R.A., Daniel-Igwe G., Amaefule, K.U. and Oguike, M.A. (2019). Relative organ weights of four-week old broilers fed low protein diets supplemented with multi-enzyme in humid tropics. Journal of Veterinary Medicine, University of Nigeria Nsukka, 9(2).
Amusa, H.O., Kehinde, R.A., Atoyebi, R.O. and Abu, O.A. (2015). Haematology, serum biochemistry, organ weight changes of Wistar rats fed processed dehulled jack bean (Canavalia ensiformis). Nigerian Journal of Animal Production, 42 (1): 71-78.
Brockus CW, Mahaffey EA, Bush SE, KruppDespain AW (2005). Hematologic and serum biochemical reference intervals for Vietnamese pot bellied pigs (Sus scrofa). Comp Clin Path 13: 162–165.
Emiola IA, Ojebiyi O, Akande TO (2011). Performance and organ weights of laying hens fed diets containing graded levels of sun-dried cocoa bean shell (CBS). International Journal of Poultry Science.10:987-990
Esonu, B.O., Ogbonna, U.D., Anyanwu, G.A., Emenalom, O.O., Uchegbu Etuk, E.B. and Udedibie, A.B.I. (2006). Evaluation of performance, organ characteristics and economic analysis of broiler fed dried rumen digester, International Journal of Poultry Science 5(2): 1116-1118.
Etim, N. N., Enyenihi, G. E., Williams, M. E., Udo, M. D. And Offiong, E. E. A. (2013). Haematological Parameters: Indicators Of The Physiological Status Of Farm Animals. British Journal Of Science, 10: 33 – 45.
Eze, J.I., Onunkwo, J.I., Shoyinka, S. V. O., Chah, F.K.,Ngene, A. A.,Okolinta, N., Nwanta, J.A. and Onyenwe, I.W.(2010). Haematological Profiles of Pigs raised under intensive management system in South-Eastern Nigeria. Nigerian veterinary Journal Vol. 31(2): 115-123
Isaac, L. J., Abah, G., Akpan, B., & Ekaette, I. U. (2013). Haematological properties of different breeds and sexes of rabbits (p.24-27). Proceedings of the 18th Annual Conference of Animal Science Association of Nigeria. ISBN 978-1-60327-502-6
John Dafaar Damsere, Michael Boateng, Kwame Owusu Amoah, Yaw Oppong Frimpong and Daniel Boye Okai (2020). The response of pigs to diets containing varying levels of cocoa placenta meal (CPM) supplemented with an exogenous enzyme complex. African Journal of Agricultural Research.
Magistrelli D, Zanchi R, Malagutti L, Galassi G, Canzi E, Rosi F (2016). Effects of cocoa husk feeding on the composition of swine intestinal microbiota. Journal of Agriculture and Food Chemistry.;64:2046-2052
Makinde, O. J., Okunade, S. A., Opoola, E., Sikiru, A. B., Ajide, S. O., and Elaigwu, S. (2019). Exploration of Cocoa (Theobroma cacao) By-Products as Valuable Potential Resources in Livestock Feeds and Feeding Systems. In Theobroma Cacao-Deploying Science for Sustainability of Global Cocoa Economy. IntechOpen.
NseAbasi N. Etim, Mary E. Williams, Uduak Akpabio, and Edem E. A. Offiong (2014). Haematological Parameters and Factors Affecting Their Values. Agricultural Science Volume 2, Issue 1, 37-47 ISSN 2291-4471 E-ISSN 2291-448X Published by Science and Education Centre of North America
Nwakolor, I. M. (2001). Crossing breeding of large white and Nsukka local pigs: genotype and age are related changes in haematological parameters. Tropical Journal of Animal Science 4(2): 85 – 96.
Oduro-Mensah, D.; Ocloo, A.; Lowor, S.T.; Mingle, C.; Okine, L.K.-A.; Adamafio, N.A. (2018). Bio-detheobromination of cocoa pod husks: reduction of ochratoxin A content without change in nutrient profile. Microb. Cell Factories 2018, 17, 79.
Olorode, B.R.; Onifade, A.A., Okpara, A.O. and Babatunde, G.M. (1996). Growth, Nutrient Retention, Hematology and Serum chemistry of Broiler Chickens fed Shea butter cake or Palm Kernel Cake in the Humid Tropics J. Appl. Anim. Res. 10”173-180.
Olayinka John Makinde, Sunday A. Okunade, Emmanuel Opoola, Akeem Babatunde Sikiru, Solomon O. Ajide and Sunday Elaigwu (2019). Exploration of Cocoa (Theobroma cacao) By Products as Valuable Potential Resources in Livestock Feeds and Feeding Systems
Olafedehan, C. O., Obun, A. M., Yusuf, M. K., Adewumi, O. O., Oladefedehan, A. O., Awofolaji, A. O., & Adeniji, A. A. (2010). Effects of residual cyanide in processed cassava peal meals on haematological and biochemical indices of growing rabbits (p.212). Proceedings of 35th Annual Conference of Nigerian Society for Animal Production.
Olumide, M.D., Hamzat, R.A., Bamijoko, O.J.3 and Akinsoyinu A.O. (2017) Effects of Treated Cocoa (Theobroma cacao) Bean Shell Based Diets on Serum Biochemistry and Haematological Indices of Laying Hens.
Omotoso, O. B., Ogunshola , O. J., Omoleye, S. O. and Alokan, J. A. (2017). Haematological And Serum Biochemical Responses Of West African Dwarf Goats Fed Panicum maximum Replaced With Untreated Cocoa Pod Husk Meal. Animal Research International.
Ogunsipe MH, Balogun KB, Oladepo AD, Ayoola MA, Arikewuyo MT (2017). Nutritive value of cocoa bean shell meal and its effect on growth and haematology of weanling rabbits. Nigerian Journal of Agriculture, Food and Environment.;13(1):23-28.
Research Animal Resource [RAR]. (2009). Reference values for laboratory animals: Normal haematological values. RAR Websites, RAR, University of Minnesota. Retrieved from http://www.ahc.umn.edu/rar/refvalues.html
Spring (2014). Integrated Hematology System. School Of Medicine and Public Health. University of Wisconsin-Madison.
Tewe, O.O. (1997). Sustainability and Development Paradigm from Nigeria’s Livestock Industry. Inaugural Lecture delivered on behalf of Faculty of Agriculture and Forestry, University of Ibadan. Pp.50.
Ukpabi, U.H., Salawa, L.A., Mbachu, C.L. and Oguike, B. C. (2015). Haematological responses of weaner pigs fed raw Tigernut (Cyperus esculentus) based diets. Proceeding of 20th Annual Conference of Animal Science Association of Nigeria. Pp 6-10.
Wikipedia (2020). http://en.m.wikipedia.org/wiki/Ejigbo